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FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit ORP150 Monoclonal Antibody | anti-ORP150 antibody
ORP150 Antibody
Gene Names
HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
ORP150; Monoclonal Antibody; ORP150 Antibody; 150 kDa oxygen regulated protein antibody; 150 kDa oxygen-regulated protein antibody; 170 kDa glucose regulated protein antibody; 170 kDa glucose-regulated protein antibody; DKFZp686N08236 antibody; FLJ94899 antibody; FLJ97572 antibody; glucose regulated protein 170 antibody; Grp 170 antibody; GRP-170 antibody; Grp170 antibody; HSP 12A antibody; HSP12A antibody; Hyou1 antibody; HYOU1_HUMAN antibody; Hypoxia up regulated 1 antibody; hypoxia up-regulated 1 precursor antibody; Hypoxia up-regulated protein 1 antibody; Hypoxia upregulated 1 antibody; Orp 150 antibody; ORP-150 antibody; Orp150 antibody; oxygen regulated protein (150kD) antibody; anti-ORP150 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG39-11
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ORP150 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:5000-1:10,000
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human ORP150 aa 800-950.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5, 000 dilution.)
WB (Western Blot)
(Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5, 000 dilution.)
Related Product Information for anti-ORP150 antibody
ORP150, also known as HYOU1 (hypoxia upregulated 1), is a 999 amino acid protein that localizes to the lumen of the endoplasmic reticulum (ER) and is a member of the heat shock protein 70 (HSP 70) family. ORP150 is highly expressed in tissues that have well-developed ERs and a large number of secretory proteins (such as liver and pancreas). It is expressed at lower levels in kidney and brain, and plays an essential role in cytoprotective cellular responses to hypoxia (oxygen deprivation). Specifically, ORP150 participates in protein folding and secretion in the ER and functions to protect cells from hypoxia-induced apoptosis, thereby playing a crucial role in cell survival. ORP150 expression is upregulated in a variety of tumors, such as breast cancer, suggesting an important role in tumorigenesis. The gene encoding ORP150 has two translation initiation sites, resulting in a truncated transcript that lacks an ER signal peptide, but is thought to function as a housekeeping protein in the cytoplasm.
Product Categories/Family for anti-ORP150 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
111,335 Da
NCBI Official Full Name
HYOU1 protein
NCBI Official Synonym Full Names
hypoxia up-regulated 1
NCBI Official Symbol
HYOU1
NCBI Official Synonym Symbols
Grp170; HSP12A; ORP150; GRP-170; ORP-150
NCBI Protein Information
hypoxia up-regulated protein 1; glucose-regulated protein 170; 150 kDa oxygen-regulated protein; oxygen regulated protein (150kD); 170 kDa glucose-regulated protein
UniProt Protein Name
Hypoxia up-regulated protein 1
UniProt Gene Name
HYOU1
UniProt Synonym Gene Names
GRP170; ORP150; ORP-150; GRP-170
UniProt Entry Name
HYOU1_HUMAN
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Product Notes
The ORP150 hyou1 (Catalog #AAA30512) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ORP150 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ORP150 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:5000-1:10,000 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ORP150 hyou1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORP150, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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