IHC (Immunohistochemisry)
(Immunohistochemistry analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2ug/ml anti-OPN 53.)
Mouse anti-Human Osteopontin Monoclonal Antibody | anti-SPP1 antibody
Osteopontin monoclonal antibody (53)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Protein G affinity purified.
Synonyms
Osteopontin, Antibody; Osteopontin monoclonal antibody (53); OPN; SPP1; Secreted phosphoprotein 1; Bone sialoprotein 1; anti-SPP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
53
Specificity
Recognizes human Osteopontin.
Purity/Purification
Protein G affinity purified.
Form/Format
Liquid. In PBS containing 0.1% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
314
Applicable Applications for anti-SPP1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant human Osteopontin.
Source
Purified from ascites
Country of Origin
US
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2ug/ml anti-OPN 53.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2ug/ml anti-OPN 53.)
SDS-PAGE
(SDS-PAGE analysis of AAA76990: Lane 1: MW Marker, Lane 2 and Lane 3: 2ug of Osteopontin monoclonal antibody (53), resolved under reduced and non-reduced condition.)
SDS-PAGE
(SDS-PAGE analysis of AAA76990: Lane 1: MW Marker, Lane 2 and Lane 3: 2ug of Osteopontin monoclonal antibody (53), resolved under reduced and non-reduced condition.)
WB (Western Blot)
(Western blot analysis: Protein samples were resolved by SDS-PAGE on a 8-16% gradient gel, transferred to nitrocellulose, and blocked for 2 hrs in Tris-buffered saline containing 0.05% Tween 20 (TBS- T) and 5% milk powder. For immunodetection, Blots were incubated with anti-OPN 53 (5ug/ml) overnight at 4°C, followed by incubation with a HRP-conjugated goat anti-mouse secondary antibody (in TBS-T) for 1 hr at room temperature. Protein bands were visualized by enhanced chemiluminescence on Kodak X-OMAT film. M= Magic Mark.)
WB (Western Blot)
(Western blot analysis: Protein samples were resolved by SDS-PAGE on a 8-16% gradient gel, transferred to nitrocellulose, and blocked for 2 hrs in Tris-buffered saline containing 0.05% Tween 20 (TBS- T) and 5% milk powder. For immunodetection, Blots were incubated with anti-OPN 53 (5ug/ml) overnight at 4°C, followed by incubation with a HRP-conjugated goat anti-mouse secondary antibody (in TBS-T) for 1 hr at room temperature. Protein bands were visualized by enhanced chemiluminescence on Kodak X-OMAT film. M= Magic Mark.)
Related Product Information for anti-SPP1 antibody
Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival. Plasma Osteopontin has been shown to provide prognostic information in breast, prostate, colon, and lung cancers as well as metastatic carcinomas. The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack. Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies.
Product Categories/Family for anti-SPP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Osteopontin
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
NCBI Protein Information
osteopontin
UniProt Protein Name
Osteopontin
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
UniProt Entry Name
OSTP_HUMAN
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Product Notes
The SPP1 spp1 (Catalog #AAA76990) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Osteopontin monoclonal antibody (53) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Osteopontin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SPP1 spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteopontin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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