Application Data
(Fig.2: CGE analysis of purified EQ-8D11-C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2ug of purified EQ-8D11-C1 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by CGE (Fig.2).)
Mouse Ovine CD34 Monoclonal Antibody | anti-CD34 antibody
anti-ovine CD34 monoclonal antibody
Applications
ELISA, Flow Cytometry
Purity
Protein G purified
Synonyms
Ovine CD34, Antibody; anti-ovine CD34 monoclonal antibody; anti-CD34 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a/kappa
Clone Number
EQ-8D11-C1
Specificity
Anti-ovine CD34
Purity/Purification
Protein G purified
Form/Format
Phosphate buffered saline, pH 7.2
Sequence Length
382
Applicable Applications for anti-CD34 antibody
Flow Cytometry (FC); ELISA; CELISA
Application Notes
Flow Cytometry: 1.2 ug/10^6 cells
ELISA: 1:200-1:400
CELISA:1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
ELISA: 1:200-1:400
CELISA:1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Immunogen
Genetic immunisation with cDNA encoding the extracellular domain of ovine CD34
Selection
Based on recognition of the complete native protein expressed on transfected mammalian cells.
Preparation and Storage
Short term: 2-8°C; long term: -20°C.
(Avoid repeated freezing and thawing)
(Avoid repeated freezing and thawing)
Application Data
(Fig.2: CGE analysis of purified EQ-8D11-C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2ug of purified EQ-8D11-C1 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by CGE (Fig.2).)
Application Data
(Fig.2: CGE analysis of purified EQ-8D11-C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2ug of purified EQ-8D11-C1 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by CGE (Fig.2).)
FCM (Flow Cytometry)
(Flow Cytometry analysis of BOSC23 cells using EQ-8D11-C1 Cat.# AAA14097. BOSC23 cells were transiently transfected with an expression vector encoding either CD34 (red curve) or an irrelevant protein (control transfectant: blue and green curves). Binding of EQ-8D11-C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CD34 transfected cells.)
FCM (Flow Cytometry)
(Flow Cytometry analysis of BOSC23 cells using EQ-8D11-C1 Cat.# AAA14097. BOSC23 cells were transiently transfected with an expression vector encoding either CD34 (red curve) or an irrelevant protein (control transfectant: blue and green curves). Binding of EQ-8D11-C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CD34 transfected cells.)
Related Product Information for anti-CD34 antibody
CD34 is a type I membrane protein with an apparent MW of approximately 115 kDa. It is aputative adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells (2). It is thought to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.
References
Shaw, SWS et al 2011: Sheep amniotic fluid derived CD34+ stem cells could engraft and restore
the hematopoietic system of NSG mice. ISSCR 9th annual meeting, Poster board #2416
Porada CD et al (2008): Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells. Exp Hematol, 36/12: 1739-1749.
Civin CI et al (1984): Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol. 133/1: 157-65.
Porada CD et al (2008): Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells. Exp Hematol, 36/12: 1739-1749.
Civin CI et al (1984): Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol. 133/1: 157-65.
NCBI and Uniprot Product Information
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Product Notes
The CD34 cd34 (Catalog #AAA14097) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ovine CD34 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC); ELISA; CELISA. Flow Cytometry: 1.2 ug/10^6 cells ELISA: 1:200-1:400 CELISA:1:200 - 1:400 For each application a titration should be performed to determine the optimal concentration. Researchers should empirically determine the suitability of the CD34 cd34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ovine CD34, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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