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WB (Western Blot)
(Detection of human p16INK4a by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, OVCAR-8, SK-MEL-28, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
p16INK4a recombinant antibody
Rabbit anti-p16INK4a Recombinant Monoclonal Antibody [BLR318M]
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
p16INK4a, Recombinant Antibody; Rabbit anti-p16INK4a Recombinant Monoclonal Antibody [BLR318M]; P16-INK4A; multiple tumor suppressor 1; P14ARF; P16; P16INK4; MTS1; P16INK4A; MTS-1; P19; P19ARF; TP16; p16-INK4; ARF; P14; alternative reading frame; MLM; CDK4 inhibitor p16-INK4; CDK4I; CDKN2; CMM2; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); INK4; INK4A; cell cycle negative regulator beta; Cyclin-dependent kinase inhibitor 2A; cyclin dependent kinase inhibitor 2A; p16INK4a recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR318M
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for p16INK4a recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 106 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human p16INK4a by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, OVCAR-8, SK-MEL-28, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human p16INK4a by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, OVCAR-8, SK-MEL-28, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human p16INK4a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707 lot 1) used for IP at 12 ul/mg lysate. p16INK4a was also immunoprecipitated by a second antibody against a different epitope of p16INK4a (RMAB3177B-3C3). For blotting immunoprecipitated p16INK4a, AAA213707 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human p16INK4a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707 lot 1) used for IP at 12 ul/mg lysate. p16INK4a was also immunoprecipitated by a second antibody against a different epitope of p16INK4a (RMAB3177B-3C3). For blotting immunoprecipitated p16INK4a, AAA213707 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry)
(Detection of human p16INK4a by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemisry)
(Detection of human p16INK4a by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human p16INK4a by immunocytochemistry. Sample: FFPE section of Hek293T cells. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human p16INK4a by immunocytochemistry. Sample: FFPE section of Hek293T cells. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human p16INK4a (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human p16INK4a (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody (AAA213707) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for p16INK4a recombinant antibody
p16INK4a is a negative regulator of CDK4 and CDK6 kinase activity and cell cycle progression. p16INK4a regulates the cell cycle by inhibiting cyclin/CDK complex formation and the phosphorylation of the retinoblastoma protein (pRb). The gene which encodes p16INK4a, CDKN2A, is frequently mutated or deleted in a wide variety of tumors and is known to be an important tumor suppressor gene. The CDKN2A gene also generates a distinct transcript variant and protein, p14ARF, from an alternate open reading frame (ARF).
Product Categories/Family for p16INK4a recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,533 Da
NCBI Official Full Name
cyclin-dependent kinase inhibitor 2A isoform p16INK4a
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol
CDKN2A
NCBI Official Synonym Symbols
ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
NCBI Protein Information
cyclin-dependent kinase inhibitor 2A; CDK4 inhibitor p16-INK4; multiple tumor suppressor 1; cell cycle negative regulator beta; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
UniProt Protein Name
Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
UniProt Gene Name
CDKN2A
UniProt Synonym Gene Names
CDKN2; MTS1; CDK4I; MTS-1; p16-INK4; p16INK4A
UniProt Entry Name
CD2A1_HUMAN
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Product Notes
The p16INK4a cdkn2a (Catalog #AAA213707) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-p16INK4a Recombinant Monoclonal Antibody [BLR318M] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p16INK4a can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the p16INK4a cdkn2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p16INK4a, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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