Mouse p27Kip1 Monoclonal Antibody | anti-CDKN1B antibody

p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody

Cat. Num. AAA62716

• Gene Names: CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
• Reactivity: Human, Monkey, Mouse, Rat.; Others not known
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: p27Kip1, Antibody; p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody; CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4; anti-CDKN1B antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, Rat.; Others not known
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: KIP1/769
• Specificity: This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 198

• Applicable Applications for anti-CDKN1B antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Nuclear
• Immunogen: Recombinant human CDKN1B protein
• Hu-Chromosome Location: 12p13.1
• Positive Control: ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Colon stained with p27 Monoclonal Antibody (KIP1/769))

FCM/FACS (Flow Cytometry)

(Flow Cytometry of human p27 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (KIP1/769).)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Cervical cancer stained with p27 Monoclonal Antibody (KIP1/769))

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Prostate cancer stained with p27 Monoclonal Antibody (KIP1/769))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p27 Monoclonal Antibody (KIP1/769))

References

Fredersdorf S et. al. Proc Natl Acad Sci 1997;94:6380-5.

NCBI and Uniprot Product Information

• NCBI GI #: 4757962
• NCBI GeneID: 1027
• NCBI Accession #: NP_004055.1
• NCBI GenBank Nucleotide #: NM_004064.4
• UniProt Accession #: P46527; Q16307; Q5U0H2; Q9BUS6
• Molecular Weight: 25-26kDa
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1B
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 1B
• NCBI Official Symbol: CDKN1B
• NCBI Official Synonym Symbols: KIP1; MEN4; CDKN4; MEN1B; P27KIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1B
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1B
• UniProt Gene Name: CDKN1B
• UniProt Synonym Gene Names: KIP1
• UniProt Entry Name: CDN1B_HUMAN