WB (Western Blot)
(Western blot analysis of lysates from C6 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AAA283138) at 1:20000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Rabbit anti-Human p38 MAPK-T180/Y182 Monoclonal Antibody | anti-MAPK14 antibody
Phospho-p38 MAPK-T180/Y182 Rabbit mAb
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
p38 MAPK-T180/Y182, Antibody; Phospho-p38 MAPK-T180/Y182 Rabbit mAb; MAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; p38 MAPK; Phospho-p38 MAPK-T180/Y182; anti-MAPK14 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
MTGYV
Applicable Applications for anti-MAPK14 antibody
ELISA, WB (Western Blot)
Cross Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T180 & Y182 of human MAPK(NP_002742.3).
Modification
Phospho 180/Y182, Phospho T180/Y182, Phospho T180/Y182, Phospho T183/Y1845
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from C6 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AAA283138) at 1:20000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of lysates from C6 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AAA283138) at 1:20000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AAA283138) at 1:20000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AAA283138) at 1:20000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of various lysates, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AAA283138) at 1:20000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of various lysates, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AAA283138) at 1:20000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Related Product Information for anti-MAPK14 antibody
This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen activated protein kinase kinases (MKKs). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Product Categories/Family for anti-MAPK14 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 29kDa/34kDa/35kDa/41kDa
Observed MW: 43kDa
Observed MW: 43kDa
UniProt Protein Name
Mitogen-activated protein kinase 11
UniProt Gene Name
MAPK11
UniProt Synonym Gene Names
PRKM11; SAPK2; SAPK2B; MAP kinase 11; MAPK 11; MAP kinase p38 beta; p38b; SAPK2b
UniProt Entry Name
MK11_HUMAN
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Product Notes
The MAPK14 mapk11 (Catalog #AAA283138) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-p38 MAPK-T180/Y182 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p38 MAPK-T180/Y182 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAPK14 mapk11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MTGYV. It is sometimes possible for the material contained within the vial of "p38 MAPK-T180/Y182, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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