Rabbit MAPK14-Y182-p Antibody | anti-MAPK14 antibody

Phospho-MAPK14-Y182-p Polyclonal Antibody

Cat. Num. AAA36726

• Gene Names: MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity Purification
• Synonyms: MAPK14-Y182-p; N/A; Phospho-MAPK14-Y182-p Polyclonal Antibody; Phospho-MAPK14-Y182: RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15 SAPK2A; p38ALPHA; anti-MAPK14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 360

• Applicable Applications for anti-MAPK14 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic phosphorylated peptide around Y182 of human MAPK (NP_620581.1).
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

WB (Western Blot)

(Western blot analysis of 293T, using Phospho-p38 MAPK-Y182 antibody (AAA36726) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AAA36726) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

Related Product Information for anti-MAPK14 antibody

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38alpha, beta, gamma (also known as Erk6 or SAPK3), and delta (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

Product Categories/Family for anti-MAPK14 antibody

Phosphorylation

NCBI and Uniprot Product Information

• NCBI GI #: 2499600
• NCBI GeneID: 1432
• UniProt Accession #: Q16539; O60776; Q13083; Q14084; Q8TDX0; A6ZJ92; A8K6P4; B0LPH0; B5TY32
• Molecular Weight: Observed MW: 42kDa; Calculated MW: 41kDa
• NCBI Official Full Name: Mitogen-activated protein kinase 14
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 14
• NCBI Official Symbol: MAPK14
• NCBI Official Synonym Symbols: RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
• NCBI Protein Information: mitogen-activated protein kinase 14; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; MAP kinase p38 alpha; CSAID-binding protein; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; p38 mitogen activated protein kinase; mitogen-activated protein kinase p38 alpha; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug-binding protein
• UniProt Protein Name: Mitogen-activated protein kinase 14
• UniProt Gene Name: MAPK14
• UniProt Synonym Gene Names: CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; MAP kinase 14; MAPK 14; CSAID-binding protein; CSBP; MAP kinase p38 alpha; SAPK2a
• UniProt Entry Name: MK14_HUMAN