Mouse p38alpha MAP Kinase Monoclonal Antibody | anti-MAPK14 antibody

p38alpha MAP Kinase (C-terminal) Antibody

Cat. Num. AAA71690

• Gene Names: MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Immunocytochemistry, Western Blot
• Purity: Protein G chromatography
• Synonyms: p38alpha MAP Kinase, Antibody; p38alpha MAP Kinase (C-terminal) Antibody; p38alpha MAP Kinase (C-terminal); p 38 M548; anti-MAPK14 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M548
• Specificity: This antibody detects a 38kDa* protein corresponding to the apparent molecular mass of p38alpha on SDS-PAGE immunoblots of human A431 and rat A7r5 cells.
• Purity/Purification: Protein G chromatography
• Form/Format: Mouse monoclonal purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-MAPK14 antibody: ELISA, ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: Clone M548 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human p38alpha. This sequence is identical in rat and mouse p38alpha.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of p38alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38alpha (PM5481). The antibody was detected using goat anti-mouse DyLight 594.)

WB (Western Blot)

(Western blot analysis of human A431 cells (lanes 1 & 2) and rat A7r5 cells (lanes 3 & 4). The blot was probed with mouse monoclonal anti-p38alpha (C-terminal) clone M548 at a dilution of 1:250 (lanes 1 & 3) or 1:1000 (lanes 2 & 4).)

Related Product Information for anti-MAPK14 antibody

p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (alpha, beta, gamma, delta) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max and MEF2.

References

Jirmanova, L. et al. (2009) Blood 113(10):2229.
Salvador, J.M. et al. (2005) Nat Immunol. 6(4):390.
Han, J. et al. (1994) Science 265:808.
Lee, J. C. et al. (1994) Nature 372:739.

NCBI and Uniprot Product Information

• NCBI GI #: 4503069
• NCBI GeneID: 1432
• NCBI Accession #: NP_001306.1
• NCBI GenBank Nucleotide #: NM_001315.2
• UniProt Accession #: Q16539; O60776; Q13083; Q14084; Q8TDX0; A6ZJ92; A8K6P4; B0LPH0
• Molecular Weight: 41,293 Da
• NCBI Official Full Name: mitogen-activated protein kinase 14 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 14
• NCBI Official Symbol: MAPK14
• NCBI Official Synonym Symbols: RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
• NCBI Protein Information: mitogen-activated protein kinase 14; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; MAP kinase p38 alpha; CSAID-binding protein; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; p38 mitogen activated p
• UniProt Protein Name: Mitogen-activated protein kinase 14
• UniProt Gene Name: MAPK14
• UniProt Synonym Gene Names: CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; MAP kinase 14; MAPK 14; CSAID-binding protein; CSBP; MAP kinase p38 alpha; SAPK2a
• UniProt Entry Name: MK14_HUMAN