ICC (Immunocytochemistry)
(Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
Rabbit p38alpha MAP Kinase Polyclonal Antibody | anti-Mapk14 antibody
p38alpha MAP Kinase (Tyr-323), phospho-specific Antibody
Gene Names
Mapk14; p38; Crk1; Mxi2; p38a; CSBP2; Csbp1; PRKM14; PRKM15; p38MAPK; p38alpha; p38-alpha
Reactivity
Human, Rat, Mouse, Chicken
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
p38alpha MAP Kinase, Antibody; p38alpha MAP Kinase (Tyr-323), phospho-specific Antibody; p38alpha MAP Kinase (Tyr-323), phospho-specific; p 38 Tyr-323; anti-Mapk14 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to a phospho-tyrosine peptide before affinity purification using phospho-p38alpha (Tyr-323) peptide (without carrier). The antibody detects a 38kDa* band corresponding to p38alpha on SDS-PAGE immunoblots of human Jurkat, K562, and mouse macrophage (J774A.1) cells treated with pervanadate. In addition, the antibody detects recombinant p38 MAP Kinase when treated with Fyn kinase (The in vitro kinase model was kindly provided by Dr. Paul Mittelstadt from the Laboratory of Immune Cell Biology at the NCI.)
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Mapk14 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Phospho-p38alpha MAP Kinase (Tyr-323) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 323 in mouse p38alpha. This peptide sequence is highly conserved in human and rat p38alpha, and has high homology to the conserved site in p38beta.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
WB (Western Blot)
(A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38alpha (Tyr-323) (lanes 3 & 4).)
WB (Western Blot)
(A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38alpha (Tyr-323) (lanes 3 & 4).)
Related Product Information for anti-Mapk14 antibody
p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (alpha, beta, gamma, delta) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.
References
Jirmanova, L. et al. (2009) Blood 113(10):2229.
Salvador, J.M. et al. (2005) Nat Immunol. 6(4):390.
Han, J. et al. (1994) Science 265:808.
Lee, J. C. et al. (1994) Nature 372:739.
Salvador, J.M. et al. (2005) Nat Immunol. 6(4):390.
Han, J. et al. (1994) Science 265:808.
Lee, J. C. et al. (1994) Nature 372:739.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
32,327 Da
NCBI Official Full Name
Mitogen-activated protein kinase 14
NCBI Official Synonym Full Names
mitogen-activated protein kinase 14
NCBI Official Symbol
Mapk14
NCBI Official Synonym Symbols
p38; Crk1; Mxi2; p38a; CSBP2; Csbp1; PRKM14; PRKM15; p38MAPK; p38alpha; p38-alpha
NCBI Protein Information
mitogen-activated protein kinase 14
UniProt Protein Name
Mitogen-activated protein kinase 14
UniProt Gene Name
Mapk14
UniProt Synonym Gene Names
Crk1; Csbp1; Csbp2; MAP kinase 14; MAPK 14; MAP kinase p38 alpha
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Product Notes
The Mapk14 mapk14 (Catalog #AAA71695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p38alpha MAP Kinase (Tyr-323), phospho-specific Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's p38alpha MAP Kinase can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Mapk14 mapk14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p38alpha MAP Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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