IHC (Immunohistochemistry)
(Ovarian high grade serous carcinoma with diffuse strong nuclear p53 expression. Formalin fixed, paraffin embedded human tissue (4 um section) stained with anti - p53 monospecific clonal antibody.)
Rabbit anti-Human p53 Monoclonal Antibody | anti-p53 antibody
Anti - p53
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
p53, Antibody; Anti - p53; anti-p53 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
M26-A
Specificity
Human
Sequence Length
393
Applicable Applications for anti-p53 antibody
IHC (Immunohistochemistry)
Immunogen
Peptide derived from N-terminal sequence of human p-53. Antibody recognizes the epitope between Ser46-Pro67.
Buffer
20 mM Tris-HCl, pH 8.0
Stabilizer
20 mg/ml BSA
Preservative
0.05% NaN3
Cellular localization
Nucleus
Positive Control
renal cell carcinoma tissue, colon carcinoma tissue.
IHC-P Protolcol
Instruction Manual
1. Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water.
6. For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, 0.05% Tween-20*, and incubate in water bath at 96-98ºC for 20-25 minutes. (Alternatively adjust to your own protocol, keeping the required pH).
7. Transfer the slide to room temperature and let it cool down (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water.
9. Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10.
Concentrated:
Incubate the section with primary antibody at the dilution 1:100-1:500 for 1 hour in the closed wet chamber.
Ready to use(RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash twice 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual of MBS, Inc. is suggested.
13. Wash twice 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1-3 minutes.
15. Rinse in water.
16. Stain in hematoxylin for 5 minutes.
17. Wash in water – 10 minutes.
18. Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.
19. Wash the section in 2 changes of xylene for 2 minutes each.
20. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris -------- 1.21 g; EDTA --------- 0.37 g; Distilled water --------- 1000 ml Mix to dissolve in 700 ml of distilled water.
Adjust pH to 9.0 with 1M HCI and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4°C for longer storage.
1. Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water.
6. For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, 0.05% Tween-20*, and incubate in water bath at 96-98ºC for 20-25 minutes. (Alternatively adjust to your own protocol, keeping the required pH).
7. Transfer the slide to room temperature and let it cool down (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water.
9. Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10.
Concentrated:
Incubate the section with primary antibody at the dilution 1:100-1:500 for 1 hour in the closed wet chamber.
Ready to use(RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash twice 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual of MBS, Inc. is suggested.
13. Wash twice 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1-3 minutes.
15. Rinse in water.
16. Stain in hematoxylin for 5 minutes.
17. Wash in water – 10 minutes.
18. Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.
19. Wash the section in 2 changes of xylene for 2 minutes each.
20. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris -------- 1.21 g; EDTA --------- 0.37 g; Distilled water --------- 1000 ml Mix to dissolve in 700 ml of distilled water.
Adjust pH to 9.0 with 1M HCI and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4°C for longer storage.
Precautions
1. We strongly recommend using the Primary Antibody Diluent , eventually alternative diluent (containing protease free BSA at the concentrations > = 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.
2. Centrifuge the vial before use.
3. Intended for research use in laboratories.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
2. Centrifuge the vial before use.
3. Intended for research use in laboratories.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Preparation and Storage
Store at +4 degree C.
IHC (Immunohistochemistry)
(Ovarian high grade serous carcinoma with diffuse strong nuclear p53 expression. Formalin fixed, paraffin embedded human tissue (4 um section) stained with anti - p53 monospecific clonal antibody.)
IHC (Immunohistochemistry)
(Ovarian high grade serous carcinoma with diffuse strong nuclear p53 expression. Formalin fixed, paraffin embedded human tissue (4 um section) stained with anti - p53 monospecific clonal antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Cellular tumor antigen p53
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; TRP53
NCBI Protein Information
cellular tumor antigen p53; antigen NY-CO-13; tumor protein 53; phosphoprotein p53; p53 tumor suppressor; mutant tumor protein 53; transformation-related protein 53
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
UniProt Entry Name
P53_HUMAN
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Product Notes
The p53 tp53 (Catalog #AAA79002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - p53 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p53 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the p53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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