WB (Western Blot)
(Anti - p53 (DB 002) Western blot of p53 in mouse brain crude lysate (50 ?g of protein loaded).)
Rabbit p53 Monoclonal Antibody | anti-p53 antibody
Anti - p53
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, mouse, rat
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot, ELISA
Synonyms
p53, Antibody; Anti - p53; anti-p53 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Monoclonal
Clone Number
S11-K
Specificity
Human, rat, mouse-tested
Sequence Length
393
Applicable Applications for anti-p53 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot), ELISA
Basic information
Major clone of rabbit immunoglobulin corresponding to immunogenic peptide
Immunogen
Peptide derived from C-terminal sequence of human p53. Antibody recognizes the epitope located between Lys372 - His380.
Storage Buffer
20 mM Tris-HCI, pH 8.0
Stabilizer
10 mg/ml BSA
Uniprot
Human: P04637; Mouse: P02340; Rat: P10361
Preservative
0.05% Sodium Azide
Uniprot
P04637; P10361; P02340
Product description
Rabbit anti-p53 clonal IgGs
IMMUNOCYTOCHEMISTRY (ICC) PROTOCOL
Coat coverslips with 1% gelatin-coating solution for 2 hours at room temperature (RT);
1.rinse with distilled water, and let to dry overnight. Before plating the cells, wash the coated coverslips briefly with PBS.
Fix the cells with 4% paraformaldehyde solution (in PBS, pH 7.2), for 15 min at RT.
2. Wash 2 x 3 min with PBS.
3. Permeabilize the cells with 0.1% Triton X-100 solution (in PBS, pH 7.2) for 5 min on ice.
4. Wash 2 x 3 min with PBS.
5. Incubate the cells in blocking buffer (0.3M glycine in PBS, 2% BSA) for 30 min at RT.
6. Incubate the cells with primary antibody: anti-p53 clonal antibody at the dilution of 1:100
7.- 1:500 in antibody dilution buffer (PBS, 1% BSA) for 1 hour at RT in humid chamber.
Wash 2 x 3 min with PBS.
8. Apply the secondary antibody (in this case, the goat anti-rabbit IgG-FITC from Jackson
9.Immunoresearch, cat. #” please inquire”, was used at 1:300 in antibody dilution buffer, and cells were incubated for 1 hour at RT in dark).
Wash 3 x 3 min with PBS.
10. Rinse once with distilled water.
11.Mount the slide for observation, with a drop of anti-fade mounting medium.
1.rinse with distilled water, and let to dry overnight. Before plating the cells, wash the coated coverslips briefly with PBS.
Fix the cells with 4% paraformaldehyde solution (in PBS, pH 7.2), for 15 min at RT.
2. Wash 2 x 3 min with PBS.
3. Permeabilize the cells with 0.1% Triton X-100 solution (in PBS, pH 7.2) for 5 min on ice.
4. Wash 2 x 3 min with PBS.
5. Incubate the cells in blocking buffer (0.3M glycine in PBS, 2% BSA) for 30 min at RT.
6. Incubate the cells with primary antibody: anti-p53 clonal antibody at the dilution of 1:100
7.- 1:500 in antibody dilution buffer (PBS, 1% BSA) for 1 hour at RT in humid chamber.
Wash 2 x 3 min with PBS.
8. Apply the secondary antibody (in this case, the goat anti-rabbit IgG-FITC from Jackson
9.Immunoresearch, cat. #” please inquire”, was used at 1:300 in antibody dilution buffer, and cells were incubated for 1 hour at RT in dark).
Wash 3 x 3 min with PBS.
10. Rinse once with distilled water.
11.Mount the slide for observation, with a drop of anti-fade mounting medium.
Preparation and Storage
Storage: 10 ul aliquots at -20 degree C
Handling: Avoid repeated freezing and thawing
Expiration: 24 months (from the date of delivery)
Handling: Avoid repeated freezing and thawing
Expiration: 24 months (from the date of delivery)
WB (Western Blot)
(Anti - p53 (DB 002) Western blot of p53 in mouse brain crude lysate (50 ?g of protein loaded).)
WB (Western Blot)
(Anti - p53 (DB 002) Western blot of p53 in mouse brain crude lysate (50 ?g of protein loaded).)
Application Data
(Representative picture of p53 expression in HEK293 cells, visualized with clonal rabbit anti-p53 monospecific antibody. Primary antibody dilution - 1:200.)
Application Data
(Representative picture of p53 expression in HEK293 cells, visualized with clonal rabbit anti-p53 monospecific antibody. Primary antibody dilution - 1:200.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
43,653 Da
NCBI Official Full Name
Cellular tumor antigen p53
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; TRP53
NCBI Protein Information
cellular tumor antigen p53; antigen NY-CO-13; tumor protein 53; phosphoprotein p53; p53 tumor suppressor; mutant tumor protein 53; transformation-related protein 53
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
UniProt Entry Name
P53_HUMAN
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Product Notes
The p53 tp53 (Catalog #AAA78999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - p53 reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's p53 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the p53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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