IF (Immunofluorescence)
(ICC/IF analysis of PARK7 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of PARK7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human PARK7 Monoclonal Antibody | anti-PARK7 antibody
PARK7 antibody
Gene Names
PARK7; DJ1; DJ-1; GATD2; HEL-S-67p
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
PARK7, Antibody; PARK7 antibody; Parkinson disease protein 7; PARK7; DJ-1 (PARK7); Oncogene DJ1; Protein DJ-1; Parkinson disease protein 7 CAP1; DJ1; DJ1 protein; SP22.; anti-PARK7 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT1E12
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
189
Applicable Applications for anti-PARK7 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human Park7/DJ-1 (1-189aa) purified from E. coli
Clone Note
Anti-human Park7/DJ-1 mAb, clone AT1E12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Park7/DJ-1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of PARK7 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of PARK7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of PARK7 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of PARK7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: NIH3T3 cell lysate Lane 4.: Mouse brain tissue lysate Lane 5.: Mouse liver tissue lysate The tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Kidney tissue lysate)
WB (Western Blot)
(The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: NIH3T3 cell lysate Lane 4.: Mouse brain tissue lysate Lane 5.: Mouse liver tissue lysate The tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Kidney tissue lysate)
Related Product Information for anti-PARK7 antibody
Parkinson disease (autosomal recessive, early onset) 7, also known as PARK7/DJ-1, has been shown to interact with EFCAB6 and protein inhibitor of activated STAT2. Defects in PARK7 are the cause of autosomal recessive early-onset Parkinson's disease 7. This protein belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death.
Product Categories/Family for anti-PARK7 antibody
References
Entrez Gene: PARK7; Bonifati V., et al. (2003) Science 299 (5604): 256-259; Takahashi K., et al. (2001) J. Biol. Chem. 276 (40): 37556-63;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,891 Da
NCBI Official Full Name
protein/nucleic acid deglycase DJ-1
NCBI Official Synonym Full Names
Parkinsonism associated deglycase
NCBI Official Symbol
PARK7
NCBI Official Synonym Symbols
DJ1; DJ-1; GATD2; HEL-S-67p
NCBI Protein Information
protein/nucleic acid deglycase DJ-1; protein deglycase DJ-1
UniProt Protein Name
Protein/nucleic acid deglycase DJ-1
UniProt Gene Name
PARK7
UniProt Synonym Gene Names
DJ-1
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Product Notes
The PARK7 park7 (Catalog #AAA48158) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PARK7 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARK7 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PARK7 park7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARK7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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