Mouse anti-Human PARP Monoclonal Antibody | anti-PARP1 antibody

Anti-PARP Antibody, Mouse Monoclonal

Cat. Num. AAA27741

• Gene Names: PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A
• Synonyms: PARP; Monoclonal Antibody; Anti-PARP Antibody; Mouse Monoclonal; PARP-1/PARP Antibody; Mouse MAb; poly (ADP-ribose) polymerase 1; Anti-ADPRT Antibody; Anti-ADPRT1 Antibody; Anti-ARTD1 Antibody; Anti-pADPRT-1 Antibody; Anti-PARP-1 Antibody; Anti-PPOL Antibody; anti-PARP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 04
• Specificity: Human PARP-1/PARP
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PARP1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:100-1:500; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human PARP-1/PARP protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PARP-1/PARP (rh PARP-1/PARP). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-PARP1 mouse monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:113 kDaObserved band size:113 kDa)

IP (Immunoprecipitation)

(PARP1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-PARP1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PARP1 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 113 kDaObserved band size: 113 kDa)

IHC (Immunohistochemistry)

(Immunochemical staining of human PARP1 in human brain with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus.)

IHC (Immunohistochemistry)

(Immunochemical staining of human PARP1 in human breast with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus.)

IHC (Immunohistochemistry)

(Immunochemical staining of human PARP1 in human lymph node with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus.)

IHC (Immunohistochemistry)

(Immunochemical staining of human PARP1 in human placenta with mouse monoclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nucleus.)

Related Product Information for anti-PARP1 antibody

Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

Product Categories/Family for anti-PARP1 antibody

Cancer Drug Targets

References

Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843. Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625. Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178. Ahell I. et al., 2008, Nature. 451: 81-85.

NCBI and Uniprot Product Information

• NCBI GI #: 156523968
• NCBI GeneID: 142
• NCBI Accession #: NP_001609.2
• NCBI GenBank Nucleotide #: NM_001618.3
• UniProt Accession #: P09874; Q8IUZ9; B1ANJ4
• Molecular Weight: 113,084 Da
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly (ADP-ribose) polymerase 1
• NCBI Official Symbol: PARP1
• NCBI Official Synonym Symbols: PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• NCBI Protein Information: poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-
• UniProt Protein Name: Poly [ADP-ribose] polymerase 1
• UniProt Gene Name: PARP1
• UniProt Synonym Gene Names: ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
• UniProt Entry Name: PARP1_HUMAN