Mouse anti-Human PARP1 / PARP Monoclonal Antibody | anti-PARP1 antibody

Mouse Monoclonal [clone A6.4.12] (IgG1) to Human PARP1 / PARP

Cat. Num. AAA51922

• Gene Names: PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry
• Purity: Protein G Purified
• Synonyms: PARP1 / PARP, Antibody; Mouse Monoclonal [clone A6.4.12] (IgG1) to Human PARP1 / PARP; Anti-PARP1 / PARP Antibody (clone A6.4.12) IHC-plus; PARP1; ADPRT; ADP-ribosyltransferase NAD(+); Adp-ribosyltransferase; ADPRT1; ARTD1; ADPRT 1; Poly(ADP-ribose) synthetase; Poly(ADP-ribosyl)transferase; PARP; Poly [ADP-ribose] polymerase 1; PADPRT-1; PARP-1; Poly (ADP-ribose) polymerase 1; Human PARP1; anti-PARP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: A6.4.12
• Specificity: Recognizes poly (ADP-ribose) polymerase 1 (PARP-1), a ~116kD nuclear enzyme cleaved during apoptosis. PARP-1, a caretaker enzyme, is involved in DNA damage repair, plays roles in diabetes pathophysiology and tumor proliferation.). As well as protecting cells from genomic instability, PARP-1 is involved in the development of both inflammatory and immune responses, and cell death by apoptosis and necrosis. Targets PARP-1, an enzyme which represents a promising target for new developments in therapeutic treatment of immune mediated diseases. PARP-1 has considerable potential for delivering selective tumor cell killing while sparing normal cells.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1014

• Applicable Applications for anti-PARP1 antibody: IF (Immunofluorescence), ELISA, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry)
• Antigen Type: Purified protein
• Immunogen: PARP1 antibody was raised against human PARP.
• Immunogen Species: PARP1 antibody was raised against Human.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. ; Microcentrifugation recommended if solution contains precipitate.

IHC (Immunohistochemisry)

(Anti-PARP antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohiostchemistry)

(Anti-PARP antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohistochemistry)

(Anti-PARP antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 156523968
• NCBI GeneID: 142
• NCBI Accession #: NP_001609.2
• NCBI GenBank Nucleotide #: NM_001618.3
• UniProt Accession #: P09874; Q8IUZ9; B1ANJ4
• Molecular Weight: 113,084 Da
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly (ADP-ribose) polymerase 1
• NCBI Official Symbol: PARP1
• NCBI Official Synonym Symbols: PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• NCBI Protein Information: poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
• UniProt Protein Name: Poly [ADP-ribose] polymerase 1
• UniProt Gene Name: PARP1
• UniProt Synonym Gene Names: ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
• UniProt Entry Name: PARP1_HUMAN