FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Cleaved PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit anti-Human PARP Monoclonal Antibody | anti-PARP antibody
Cleaved PARP Antibody
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
PARP, Antibody; Cleaved PARP Antibody; ADP-ribosyltransferase diphtheria toxin-like 1 antibody; ADPRT 1 antibody; ADPRT antibody; ADPRT1 antibody; APOPAIN antibody; ARTD1 antibody; NAD (+) ADP-ribosyltransferase 1 antibody; PARP antibody; PARP-1 antibody; PARP1 antibody; PARP1_HUMAN antibody; Poly [ADP-ribose] polymerase 1 antibody; Poly ADP ribose polymerase 1 antibody; Poly[ADP-ribose] synthase 1 antibody; PPOL antibody; SCA1 antibody; anti-PARP antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SU0314
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PARP antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Cleaved PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Cleaved PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
WB (Western Blot)
(Western blot analysis of Cleaved PARP on diferent lysates using anti-Cleaved PARP antibody at 1/1, 000 dilution. Positive control�� Lane1: Jurkat Lane2: A549)
WB (Western Blot)
(Western blot analysis of Cleaved PARP on diferent lysates using anti-Cleaved PARP antibody at 1/1, 000 dilution. Positive control�� Lane1: Jurkat Lane2: A549)
Related Product Information for anti-PARP antibody
Poly (ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD (+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly (ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity. This antibody recognizes the apoptosis-specific 89 kDa catalytic domain fragment, but it does not recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment.
Product Categories/Family for anti-PARP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113,084 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase 1
NCBI Official Symbol
PARP1
NCBI Official Synonym Symbols
PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
NCBI Protein Information
poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-
UniProt Protein Name
Poly [ADP-ribose] polymerase 1
UniProt Gene Name
PARP1
UniProt Synonym Gene Names
ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
UniProt Entry Name
PARP1_HUMAN
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Product Notes
The PARP parp1 (Catalog #AAA312201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cleaved PARP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PARP parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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