Mouse anti-Human PAX5/BSAP Monoclonal Antibody | anti-PAX5/BSAP antibody

PAX5/BSAP (Early B-Cell Marker)

Cat. Num. AAA215125

• Gene Names: PAX5; ALL3; BSAP
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: PAX5/BSAP, Antibody; PAX5/BSAP (Early B-Cell Marker); B cell lineage specific activator; BSAP; EBB-1; KLP; Paired box 5; anti-PAX5/BSAP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: PAX5/3735
• Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 21,000, full-length human proteins. The PAX5 gene is a member of thepaired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as thepaired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development andspermatogenesis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 391

• Applicable Applications for anti-PAX5/BSAP antibody: IHC (Immunohistochemistry)
• Positive Control: Daudi or Ramos cells. Tonsil, spleen, Lymph Node or Colon Carcinoma.
• Immunogen: A recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary)
• Cellular Localization: Nuclear
• Hu-chromosome Location: 9p13.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 21,000 full-length human proteins using PAX5 Mouse Monoclonal Antibody (PAX5/3735) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with PAX5 Mouse Monoclonal Antibody (PAX5/3735).)

References

Desouki MM, Post GR, Cherry D, Lazarchick J. PAX-5: a valuable immunohistochemical marker in the differential diagnosis of lymphoid neoplasms. Clin Med Res. 2010 Jul;8(2):84-8.

NCBI and Uniprot Product Information

• NCBI GI #: 9951920
• NCBI GeneID: 5079
• NCBI Accession #: NP_057953.1
• NCBI GenBank Nucleotide #: NP_057953.1
• UniProt Accession #: Q02548
• Molecular Weight: 42kDa
• NCBI Official Full Name: paired box protein Pax-5 isoform 1
• NCBI Official Synonym Full Names: paired box 5
• NCBI Official Symbol: PAX5
• NCBI Official Synonym Symbols: ALL3; BSAP
• NCBI Protein Information: paired box protein Pax-5
• UniProt Protein Name: Paired box protein Pax-5
• UniProt Gene Name: PAX5
• UniProt Synonym Gene Names: BSAP
• UniProt Entry Name: PAX5_HUMAN