FCM/FACS (Flow Cytometry)
(Fig-2: Intracellular flow analysis of Pax8 in Raji cells using 0.5 ug/10^6 cells of antibody (Clone: ABM5F33). Green represents isotype control; red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Mouse anti-Human PAX8 Monoclonal Antibody | anti-PAX8 antibody
Monoclonal Antibody to PAX8 (Clone: ABM5F33)
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
PurifiedProtein G Chromatography
Synonyms
PAX8, Antibody; Monoclonal Antibody to PAX8 (Clone: ABM5F33); Paired box protein Pax-8; anti-PAX8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
ABM5F33
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
450
Applicable Applications for anti-PAX8 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry)
Immunogen Information
A partial length recombinant protein (a.a 28-261) of PAX8 was used as the immunogen for this antibody.
Antigen
PAX8
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
FCM/FACS (Flow Cytometry)
(Fig-2: Intracellular flow analysis of Pax8 in Raji cells using 0.5 ug/10^6 cells of antibody (Clone: ABM5F33). Green represents isotype control; red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Fig-2: Intracellular flow analysis of Pax8 in Raji cells using 0.5 ug/10^6 cells of antibody (Clone: ABM5F33). Green represents isotype control; red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
WB (Western Blot)
(Fig-1: Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 0.5 ug/ml on recombinant and 2 ug/ml on Raji lysate.)
WB (Western Blot)
(Fig-1: Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 0.5 ug/ml on recombinant and 2 ug/ml on Raji lysate.)
Product Categories/Family for anti-PAX8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,044 Da
NCBI Official Full Name
Paired box protein Pax-8
NCBI Official Synonym Full Names
paired box 8
NCBI Official Symbol
PAX8
NCBI Protein Information
paired box protein Pax-8
UniProt Protein Name
Paired box protein Pax-8
UniProt Gene Name
PAX8
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Product Notes
The PAX8 pax8 (Catalog #AAA78283) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to PAX8 (Clone: ABM5F33) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAX8 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PAX8 pax8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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