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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human, Mouse PD-L1/PDCD1LG1/CD274/B7-H1 Monoclonal Antibody | anti-CD274 antibody
PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified Ab conjugated to Biotin
Synonyms
PD-L1/PDCD1LG1/CD274/B7-H1, Antibody; PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target); B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1; anti-CD274 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
PDL1/2746
Specificity
PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
Purity/Purification
Purified Ab conjugated to Biotin
Form/Format
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Sequence Length
176
Applicable Applications for anti-CD274 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Recombinant fragment (around aa 39-191) of Human CD274 (PD-L1) protein (exact sequence is proprietary)
Cellular Localization
Cell Surfaceand Cytoplasmic
Hu-chromosome Location
9p24.1
Positive Control
Jurkat, RAW, HEK293 or HepG2 cells. Human lung SqCC, cervical or breast carcinoma.
Preparation and Storage
Antibody with azide: store at 4 to 8 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746) (unconjugated). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746) (unconjugated). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human lung carcinoma stained with Biotin-conjugated PD-L1 Mouse Monoclonal Antibody (PDL1/2746).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human lung carcinoma stained with Biotin-conjugated PD-L1 Mouse Monoclonal Antibody (PDL1/2746).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human lung carcinoma stained with Biotin-conjugated PD-L1 Mouse Monoclonal Antibody (PDL1/2746).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human lung carcinoma stained with Biotin-conjugated PD-L1 Mouse Monoclonal Antibody (PDL1/2746).)
References
Dong, H., et al. 1999. Nat. Med. 5: 1365-1369.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37-50kDa
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
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Product Notes
The CD274 cd274 (Catalog #AAA215482) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PD-L1/PDCD1LG1/CD274/B7-H1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD274 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-L1/PDCD1LG1/CD274/B7-H1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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