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WB (Western Blot)
(Detection of human PDGFR beta by western blot. Samples: Whole cell lysate (50 ug) from BJ, HEK293T, GaMG, A-549, U2OS, MCF-7, IMR-90, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-eEF2 .)
PDGFR beta Recombinant Antibody | PDGFRbeta recombinant antibody
Rabbit anti-PDGFR beta Recombinant Monoclonal Antibody [BLR081G]
Gene Names
PDGFRB; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
PDGFR beta, Recombinant Antibody; Rabbit anti-PDGFR beta Recombinant Monoclonal Antibody [BLR081G]; CD140B; platelet-derived growth factor receptor 1; PENTT; PDGFR-beta; PDGF-R-beta; PDGFR-1; PDGFR1; PDGFR; NDEL1-PDGFRB; KOGS; JTK12; IBGC4; CD140 antigen-like family member B; CD antigen CD140b; beta-type platelet-derived growth factor receptor; Beta platelet-derived growth factor receptor; Activated tyrosine kinase PDGFRB; platelet-derived growth factor receptor, beta polypeptide; IMF1; platelet-derived growth factor receptor beta; Platelet-derived growth factor receptor beta; platelet derived growth factor receptor beta; PDGFRbeta recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR081G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for PDGFRbeta recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 33-532 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PDGFR beta by western blot. Samples: Whole cell lysate (50 ug) from BJ, HEK293T, GaMG, A-549, U2OS, MCF-7, IMR-90, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-eEF2 .)
WB (Western Blot)
(Detection of human PDGFR beta by western blot. Samples: Whole cell lysate (50 ug) from BJ, HEK293T, GaMG, A-549, U2OS, MCF-7, IMR-90, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-eEF2 .)
IP (Immunoprecipitation)
(Detection of human PDGFR beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1) used for IP at 6 ul per reaction. PDGFR beta was also immunoprecipitated by rabbit anti-PDGFR beta recombinant monoclonal antibody [BL-1184-1E8]. For blotting immunoprecipitated PDGFR beta, AAA213565 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PDGFR beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1) used for IP at 6 ul per reaction. PDGFR beta was also immunoprecipitated by rabbit anti-PDGFR beta recombinant monoclonal antibody [BL-1184-1E8]. For blotting immunoprecipitated PDGFR beta, AAA213565 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
ICC (Immunocytochemistry)
(Detection of human PDGFR beta in FFPE OVCAR-8 cells by ICC. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human PDGFR beta in FFPE OVCAR-8 cells by ICC. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human PDGFR beta in FFPE A-172 cells by ICC. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human PDGFR beta in FFPE A-172 cells by ICC. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human PDGFR beta (shaded) in OVCAR8 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human PDGFR beta (shaded) in OVCAR8 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213565) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for PDGFRbeta recombinant antibody
Platelet derived growth factor receptor beta is a cell surface tyrosine kinase receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB. These growth factors are members of the platelet-derived growth factor family and are mitogens for cells of mesenchymal origin. PDGFR beta is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. [taken from NCBI Entrez Gene (Gene ID: 5159)].
Product Categories/Family for PDGFRbeta recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123,968 Da
NCBI Official Full Name
platelet-derived growth factor receptor beta
NCBI Official Synonym Full Names
platelet-derived growth factor receptor, beta polypeptide
NCBI Official Symbol
PDGFRB
NCBI Official Synonym Symbols
IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
NCBI Protein Information
platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta-type platelet-derived growth factor receptor
UniProt Protein Name
Platelet-derived growth factor receptor beta
UniProt Gene Name
PDGFRB
UniProt Synonym Gene Names
PDGFR; PDGFR1; PDGF-R-beta; PDGFR-beta; PDGFR-1
UniProt Entry Name
PGFRB_HUMAN
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Product Notes
The PDGFRbeta pdgfrb (Catalog #AAA213565) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PDGFR beta Recombinant Monoclonal Antibody [BLR081G] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDGFR beta can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PDGFRbeta pdgfrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGFR beta, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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