Mouse PDK1 Monoclonal Antibody | anti-PDK1 antibody

PDK1 (C-terminal region) Antibody

Cat. Num. AAA71682

• Gene Names: PDPK1; PDK1; PDPK2; PRO0461
• Reactivity: Human, Rat, Mouse
• Applications: Immunocytochemistry, ELISA, Western Blot
• Purity: Protein A chromatography
• Synonyms: PDK1, Antibody; PDK1 (C-terminal region) Antibody; PDK1 (C-terminal region); PDK1 002; anti-PDK1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M146
• Specificity: The purified antibody detects a 65kDa* protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human A431 cells and mouse brain.
• Purity/Purification: Protein A chromatography
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-PDK1 antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: Clone M146 was generated from a recombinant protein containing amino acid residues in the C-terminal region of human PDK1. This sequence is highly conserved in rat and mouse PDK1
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.)

WB (Western Blot)

(Western blot image of mouse brain (lane 1) and human A431 cells (lane 2). The blot was probed with anti-PDK1 (C-terminal region) (PM1461).)

Related Product Information for anti-PDK1 antibody

3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survival kinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs. PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstream kinases, like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cell survival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid second messengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation is required for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylation may also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 are important for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.

References

Scheid, M.P. et al. (2005) Mol. Cell. Biol. 25(6):2347.
Taniyama, Y. et al. (2003) Mol Cell. Biol. 23(22):8019.
Park, J. et al. (2001) J Biol. Chem. 276(40):37459.

NCBI and Uniprot Product Information

• NCBI GI #: 387849239
• NCBI GeneID: 5170
• NCBI Accession #: NP_001248745.1
• NCBI GenBank Nucleotide #: NM_001261816.1
• UniProt Accession #: O15530; Q59EH6; Q6FI20; Q8IV52; Q9BRD5; H0Y4Z0; Q6A1A2
• Molecular Weight: 63,152 Da
• NCBI Official Full Name: 3-phosphoinositide-dependent protein kinase 1 isoform 3
• NCBI Official Synonym Full Names: 3-phosphoinositide dependent protein kinase-1
• NCBI Official Symbol: PDPK1
• NCBI Official Synonym Symbols: PDK1; PDPK2; PRO0461
• NCBI Protein Information: 3-phosphoinositide-dependent protein kinase 1; PkB-like 1; PkB kinase like gene 1
• UniProt Protein Name: 3-phosphoinositide-dependent protein kinase 1
• UniProt Gene Name: PDPK1
• UniProt Synonym Gene Names: PDK1; hPDK1
• UniProt Entry Name: PDPK1_HUMAN