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product-image-AAA224756_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Phospho-Ser241) antibody (left) or the same antibody preincubated with with blocking peptide (right))

Rabbit PDK1 Polyclonal Antibody | anti-PDK1 antibody

PDK1 antibody

Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified by immunoaffinity chromatography.
Synonyms
PDK1, Antibody; PDK1 antibody; Rabbit polyclonal PDK1 antibody; Polyclonal PDK1 antibody; Anti-PDK1 antibody; Pyruvate dehydrogenase kinase isoform 1 antibody; isozyme 1 antibody; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody; isozyme 1 HGNC:8809 antibody; Isoenzyme 1 antibody; PDK1; PDK-1; Pyruvate dehydrogenase kinase antibody; PDK 1; PDK-1 antibody; PDK 1 antibody; Phospho Ser241 antibody; anti-PDK1 antibody
Ordering
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
129
Applicable Applications for anti-PDK1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence)
Immunogen
PDK1 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1 as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Phospho-Ser241) antibody (left) or the same antibody preincubated with with blocking peptide (right))

product-image-AAA224756_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Phospho-Ser241) antibody (left) or the same antibody preincubated with with blocking peptide (right))

WB (Western Blot)

(Western Blot analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1 (Phospho-Ser241) antibody)

product-image-AAA224756_WB13.jpg WB (Western Blot) (Western Blot analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1 (Phospho-Ser241) antibody)

IF (Immunofluorescence)

(Immunofluorescence staining of methanol-fixed Hela cells using PDK1 (Phospho-Ser241) antibody)

product-image-AAA224756_IF15.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PDK1 (Phospho-Ser241) antibody)
Related Product Information for anti-PDK1 antibody
PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth.
Product Categories/Family for anti-PDK1 antibody

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
15,124 Da
NCBI Official Full Name
PDK1, partial
UniProt Protein Name
PDK1
UniProt Gene Name
PDK1

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Product Notes

The PDK1 pdk1 (Catalog #AAA224756) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDK1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the PDK1 pdk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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