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WB (Western Blot)
(PDK2 Antibody (Cat.# AAA283436) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).)
Mouse anti-Human, mouse PDK2 Monoclonal Antibody | anti-PDK2 antibody
PDK2 Antibody
Gene Names
PDK2; PDHK2; PDKII
Reactivity
Human, mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Synonyms
PDK2, Antibody; PDK2 Antibody; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII; PDK2; PDHK2; anti-PDK2 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Monoclonal
Isotype
IgG1,K
Clone Number
180CT10.2.3
Specificity
This PDK2 monoclonal antibody is generated from mouse immunized with PDK2 recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
343
Applicable Applications for anti-PDK2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(PDK2 Antibody (Cat.# AAA283436) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).)
WB (Western Blot)
(PDK2 Antibody (Cat.# AAA283436) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from human skeletal muscle, human brain, mouse brain, rat brain tissue (from left to right), using PDK2 Antibody(Cat.# AAA283436).AAA283436 was diluted at 1:2000 at each lane.A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from human skeletal muscle, human brain, mouse brain, rat brain tissue (from left to right), using PDK2 Antibody(Cat.# AAA283436).AAA283436 was diluted at 1:2000 at each lane.A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
IHC (Immunohistochemisry)
(AAA283436 staining PDK2 in human liver sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections).Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA283436 staining PDK2 in human liver sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections).Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
WB (Western Blot)
(All lanes : Anti- at 1:1000 dilutionLane 1: human heart lysateLane 2: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti- at 1:1000 dilutionLane 1: human heart lysateLane 2: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-PDK2 Antibody at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-PDK2 Antibody at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PDK2 antibody
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Product Categories/Family for anti-PDK2 antibody
References
Li, J., et al. J. Biol. Chem. 284(49):34458-34467(2009)
Fencl, F., et al. Pediatr. Nephrol. 24(5):983-989(2009)
Sun, W., et al. Clin. Cancer Res. 15(2):476-484(2009)
Hiromasa, Y., et al. Biochemistry 47(8):2312-2324(2008)
Hiromasa, Y., et al. Biochemistry 47(8):2298-2311(2008)
Fencl, F., et al. Pediatr. Nephrol. 24(5):983-989(2009)
Sun, W., et al. Clin. Cancer Res. 15(2):476-484(2009)
Hiromasa, Y., et al. Biochemistry 47(8):2312-2324(2008)
Hiromasa, Y., et al. Biochemistry 47(8):2298-2311(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
pyruvate dehydrogenase kinase, isozyme 2 isoform 2
NCBI Official Synonym Full Names
pyruvate dehydrogenase kinase, isozyme 2
NCBI Official Symbol
PDK2
NCBI Official Synonym Symbols
PDHK2; PDKII
NCBI Protein Information
pyruvate dehydrogenase kinase, isozyme 2
UniProt Protein Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
UniProt Gene Name
PDK2
UniProt Synonym Gene Names
PDHK2; PDH kinase 2; PDKII
UniProt Entry Name
PDK2_HUMAN
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Product Notes
The PDK2 pdk2 (Catalog #AAA283436) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PDK2 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDK2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PDK2 pdk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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