Mouse anti-Human, Dog PECAM-1 / CD31 Monoclonal Antibody | anti-PECAM-1 antibody

Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31

Cat. Num. AAA52533

• Gene Names: PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Reactivity: Human, Dog
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Immunoaffinity purified from tissue culture supernatant
• Synonyms: PECAM-1 / CD31, Antibody; Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31; PECAM1; CD31; CD31 antigen; EndoCAM; PECA1; PECAM-1; GPIIA; anti-PECAM-1 antibody

• Host: Mouse
• Reactivity: Human, Dog
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1H6
• Specificity: Human CD31
• Purity/Purification: Immunoaffinity purified from tissue culture supernatant
• Form/Format: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PECAM-1 antibody: FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Target: PECAM-1/CD31
• Immunogen Species: PECAM-1 / CD31 antibody was raised against Human.
• Antigen Type: Recombinant protein
• Immunogen: PECAM/CD31 antibody was raised against full length human recombinant protein of human PECAM (NP_00433) produced in HEK293T cell.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip.
• Preparation and Storage: Store at -20°C. Avoid freeze-thaw cycles.

WB (Western Blot)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.)

WB (Western Blot)

(Western blot of extracts (35 ug) from 9 different cell lines by using g anti-PECAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)

FCM/FACS (Flow Cytometry)

(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PECAM1 antibody, and then analyzed by flow cytometry.)

IF (Immunofluorescence)

(Anti-PECAM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1.)

IHC (Immunohistochemistry)

(Anti-PECAM-1 / CD31 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Related Product Information for anti-PECAM-1 antibody

CD31 (PECAM1) is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

NCBI and Uniprot Product Information

• NCBI GI #: 110347451
• NCBI GeneID: 5175
• NCBI Accession #: NP_000433.3
• NCBI GenBank Nucleotide #: NM_000442.3
• UniProt Accession #: P16284; Q6LDA9; Q8TBH1; Q96RF5; Q96RF6; Q9NP65; Q9NPB7; Q9NPG9; Q9NQS9; Q9NQT0; A8K3S7; D3DU31
• NCBI Official Full Name: platelet endothelial cell adhesion molecule
• NCBI Official Synonym Full Names: platelet/endothelial cell adhesion molecule 1
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• NCBI Protein Information: platelet endothelial cell adhesion molecule; CD31 antigen
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: PECAM1
• UniProt Synonym Gene Names: PECAM-1
• UniProt Entry Name: PECA1_HUMAN