FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit PGK1 Monoclonal Antibody | anti-PGK1 antibody
PGK1 Antibody
Gene Names
PGK1; PGKA; MIG10; HEL-S-68p
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
PGK1, Antibody; PGK1 Antibody; Cell migration-inducing gene 10 protein antibody; Epididymis secretory sperm binding protein Li 68p antibody; HEL S 68p antibody; MGC117307 antibody; MGC8947 antibody; MIG10 antibody; pgk1 antibody; PGK1_HUMAN antibody; PGKA antibody; Phosphoglycerate kinase 1 antibody; Primer recognition protein 2 antibody; PRP 2 antibody; anti-PGK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
ST49-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PGK1 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining PGK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PGK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot)
(Western blot analysis of PGK1 on different lysates using anti-PGK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: MCF-7)
WB (Western Blot)
(Western blot analysis of PGK1 on different lysates using anti-PGK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: MCF-7)
Related Product Information for anti-PGK1 antibody
Phosphoglycerate kinases 1/2 (PGK1/2, ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1, 3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq21.1, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis.
Product Categories/Family for anti-PGK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
417
NCBI Official Full Name
Phosphoglycerate kinase 1
NCBI Official Synonym Full Names
phosphoglycerate kinase 1
NCBI Official Symbol
PGK1
NCBI Official Synonym Symbols
PGKA; MIG10; HEL-S-68p
NCBI Protein Information
phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p
UniProt Protein Name
Phosphoglycerate kinase 1
UniProt Gene Name
PGK1
UniProt Synonym Gene Names
PGKA; PRP 2
UniProt Entry Name
PGK1_HUMAN
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Product Notes
The PGK1 pgk1 (Catalog #AAA312234) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGK1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PGK1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PGK1 pgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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