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FCM/FACS (Flow Cytometry)
(PGK1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit PGK1 Polyclonal Antibody | anti-PGK1 antibody
PGK1 Antibody (Center)
Gene Names
PGK1; PGKA; MIG10; HEL-S-68p
Reactivity
Reactivity: Human(Predicted: Horse, Monkey,Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PGK1, Antibody; PGK1 Antibody (Center); Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primerrecognition protein 2, PRP 2, PGK1, PGKA; anti-PGK1 antibody
Host
Rabbit
Reactivity
Reactivity: Human(Predicted: Horse, Monkey,Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PGK1 antibody is generated from rabbitsimmunized with a KLH conjugated syntheticpeptide between 117-145 amino acids fromthe Central region of human PGK1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBSwith 0.09% (W/V) sodium azide. This antibodyis prepared by Saturated Ammonium Sulfate(SAS) precipitation followed by dialysisagainst PBS.
Sequence
117-145
Applicable Applications for anti-PGK1 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Function
Catalyzes one of the two ATP producingreactions in the glycolytic pathway via thereversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate(PubMed: , PubMed:7391028). Inaddition to its role as a glycolytic enzyme, itseems that PGK-1 acts as a polymerase alphacofactor protein (primer recognition protein)(PubMed: ). May play arole in sperm motility (PubMed: ).
Cellular Location
Cytoplasm.
Tissue Location
Mainly expressed in spermatogonia. Localizedon the principle piece in the sperm (at proteinlevel). Expression significantly decreased inthe testis of elderly men
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PGK1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PGK1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PGK1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PGK1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (G132). AAA287052 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (G132). AAA287052 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using PGK1 Antibody (Center). AAA287052 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using PGK1 Antibody (Center). AAA287052 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Related Product Information for anti-PGK1 antibody
Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
Product Categories/Family for anti-PGK1 antibody
References
Lay, A. J., et al. Nature 408: 869-873 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
phosphoglycerate kinase 1
NCBI Official Synonym Full Names
phosphoglycerate kinase 1
NCBI Official Symbol
PGK1
NCBI Official Synonym Symbols
PGKA; MIG10; HEL-S-68p
NCBI Protein Information
phosphoglycerate kinase 1
UniProt Protein Name
Phosphoglycerate kinase 1
UniProt Gene Name
PGK1
UniProt Synonym Gene Names
PGKA; PRP 2
UniProt Entry Name
PGK1_HUMAN
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Product Notes
The PGK1 pgk1 (Catalog #AAA287052) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGK1 Antibody (Center) reacts with Reactivity: Human(Predicted: Horse, Monkey,Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PGK1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PGK1 pgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 117-145. It is sometimes possible for the material contained within the vial of "PGK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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