FCM/FACS (Flow Cytometry)
(Overlay histogram showing Jurkat cells stained with Anti-PGK1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Anti-PGK1 Antibody for 60 min at 37 °C. The secondary antibody used was Goat Anti-Mouse IgG (H&L) - AF488 at 1/200 dilution for 40 min at 37 °C. Isotype control antibody (blue line) was mouse IgG2a (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Mouse anti-Human, Mouse PGK1 Monoclonal Antibody | anti-PGK1 antibody
Anti-PGK1 Antibody
Gene Names
PGK1; PGKA; MIG10; HEL-S-68p
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
PGK1, Antibody; Anti-PGK1 Antibody; anti-PGK1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Specificity
Recognizes endogenous levels of PGK1 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-PGK1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Jurkat cells stained with Anti-PGK1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Anti-PGK1 Antibody for 60 min at 37 °C. The secondary antibody used was Goat Anti-Mouse IgG (H&L) - AF488 at 1/200 dilution for 40 min at 37 °C. Isotype control antibody (blue line) was mouse IgG2a (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Jurkat cells stained with Anti-PGK1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Anti-PGK1 Antibody for 60 min at 37 °C. The secondary antibody used was Goat Anti-Mouse IgG (H&L) - AF488 at 1/200 dilution for 40 min at 37 °C. Isotype control antibody (blue line) was mouse IgG2a (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of PGK1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of PGK1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of PGK1 expression in HEK293T (A), A431 (B), mouse brain (C) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 44 kD))
WB (Western Blot)
(Western blot analysis of PGK1 expression in HEK293T (A), A431 (B), mouse brain (C) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 44 kD))
Related Product Information for anti-PGK1 antibody
Mouse monoclonal antibody to PGK1
Product Categories/Family for anti-PGK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
417
NCBI Official Full Name
Phosphoglycerate kinase 1
NCBI Official Synonym Full Names
phosphoglycerate kinase 1
NCBI Official Symbol
PGK1
NCBI Official Synonym Symbols
PGKA; MIG10; HEL-S-68p
NCBI Protein Information
phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p
UniProt Protein Name
Phosphoglycerate kinase 1
UniProt Gene Name
PGK1
UniProt Synonym Gene Names
PGKA; PRP 2
UniProt Entry Name
PGK1_HUMAN
Similar Products
Product Notes
The PGK1 pgk1 (Catalog #AAA265931) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PGK1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PGK1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PGK1 pgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.