ICC (Immunocytochemistry)
(1. Coat coverslips with 1 % gelatin-coating solution for 2 hours at room temperature (RT): rinse with distilled water, and let to dry overnight. Before plating the cells, wash the coated coverslips briefly with PBS.2. Fix the cells with 4% paraformaldehyde solution (in PBS, pH 7.2), for 15 min at RT.3. Wash 2 x 3 min with PBS.4. Permeabilize the cells with 0.1% Triton X-100 solution (in PBS. pH 7.2) for 5 min on ice.5. Wash 2 x 3 min with PBS.6. Incubate the cells in blocking buffer (0.3M glycine in PBS, 2% BSA) for 30 min at RT.7. Incubate the cells with primary antibody: anti-phospho Erk 1,2 clonal antibody at the dilution of 1:100 - 1 :400 in antibody dilution buffer (PBS, 1 % BSA) for 1 hour at RT in humid chamber.8. Wash 2 x 3 min with PBS.9. Apply the secondary antibody (in this case, the goat anti-rabbit IgG-FITC from Jackson Immunoresearch, cat. # 111-095-003, was used at 1 :300 in antibody dilution buffer, and cells were incubated for 1 hour at RT in dark).10. Wash 3 x 3 min with PBS.11. Rinse once with distilled water.12. Mount the slide for observation, with a drop of anti-fade mounting medium.)
Rabbit PHOSPHO-Erk 1,2 Monoclonal Antibody | anti-PHOSPHO-Erk 1,2 antibody
Anti - PHOSPHO-Erk 1,2
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
Reactivity
Human, MouseTested: Rat
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot, ELISA
Synonyms
PHOSPHO-Erk 1,2, Antibody; Anti - PHOSPHO-Erk 1,2; anti-PHOSPHO-Erk 1,2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Tested: Rat
Tested: Rat
Clonality
Monoclonal
Clone Number
G15-B
Sequence Length
360
Applicable Applications for anti-PHOSPHO-Erk 1,2 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot), ELISA
Immunogen
Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk 1,2
Storage Buffer
20 mM Tris-HCI, pH 8.0
Stabilizer
10 mg/ml BSA
Preservative
0.05% NaN3
Preparation and Storage
Store at -20 degree C
ICC (Immunocytochemistry)
(1. Coat coverslips with 1 % gelatin-coating solution for 2 hours at room temperature (RT): rinse with distilled water, and let to dry overnight. Before plating the cells, wash the coated coverslips briefly with PBS.2. Fix the cells with 4% paraformaldehyde solution (in PBS, pH 7.2), for 15 min at RT.3. Wash 2 x 3 min with PBS.4. Permeabilize the cells with 0.1% Triton X-100 solution (in PBS. pH 7.2) for 5 min on ice.5. Wash 2 x 3 min with PBS.6. Incubate the cells in blocking buffer (0.3M glycine in PBS, 2% BSA) for 30 min at RT.7. Incubate the cells with primary antibody: anti-phospho Erk 1,2 clonal antibody at the dilution of 1:100 - 1 :400 in antibody dilution buffer (PBS, 1 % BSA) for 1 hour at RT in humid chamber.8. Wash 2 x 3 min with PBS.9. Apply the secondary antibody (in this case, the goat anti-rabbit IgG-FITC from Jackson Immunoresearch, cat. # 111-095-003, was used at 1 :300 in antibody dilution buffer, and cells were incubated for 1 hour at RT in dark).10. Wash 3 x 3 min with PBS.11. Rinse once with distilled water.12. Mount the slide for observation, with a drop of anti-fade mounting medium.)
ICC (Immunocytochemistry)
(1. Coat coverslips with 1 % gelatin-coating solution for 2 hours at room temperature (RT): rinse with distilled water, and let to dry overnight. Before plating the cells, wash the coated coverslips briefly with PBS.2. Fix the cells with 4% paraformaldehyde solution (in PBS, pH 7.2), for 15 min at RT.3. Wash 2 x 3 min with PBS.4. Permeabilize the cells with 0.1% Triton X-100 solution (in PBS. pH 7.2) for 5 min on ice.5. Wash 2 x 3 min with PBS.6. Incubate the cells in blocking buffer (0.3M glycine in PBS, 2% BSA) for 30 min at RT.7. Incubate the cells with primary antibody: anti-phospho Erk 1,2 clonal antibody at the dilution of 1:100 - 1 :400 in antibody dilution buffer (PBS, 1 % BSA) for 1 hour at RT in humid chamber.8. Wash 2 x 3 min with PBS.9. Apply the secondary antibody (in this case, the goat anti-rabbit IgG-FITC from Jackson Immunoresearch, cat. # 111-095-003, was used at 1 :300 in antibody dilution buffer, and cells were incubated for 1 hour at RT in dark).10. Wash 3 x 3 min with PBS.11. Rinse once with distilled water.12. Mount the slide for observation, with a drop of anti-fade mounting medium.)
WB (Western Blot)
(Western Immunoblottlng solutions:Wash buffer: 1x Tris Buffered Saline (TBS): 0.1% Triton X-100Blocking buffer: 1xTBS: 0.1% Triton X-100: 5% BSA (used with the primary antibody))
WB (Western Blot)
(Western Immunoblottlng solutions:Wash buffer: 1x Tris Buffered Saline (TBS): 0.1% Triton X-100Blocking buffer: 1xTBS: 0.1% Triton X-100: 5% BSA (used with the primary antibody))
Related Product Information for anti-PHOSPHO-Erk 1,2 antibody
Rabbit anti-phospho -Erk 1,2 clonal IgGs
Basic Information: Major clone of IgGs representing both, Erk1 and Erk2, obtained from immunoglobulins corresponding to pTE-pY dual phosphorylation sequence
Basic Information: Major clone of IgGs representing both, Erk1 and Erk2, obtained from immunoglobulins corresponding to pTE-pY dual phosphorylation sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,390 Da
NCBI Official Full Name
Mitogen-activated protein kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 1
NCBI Official Symbol
MAPK1
NCBI Official Synonym Symbols
ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
NCBI Protein Information
mitogen-activated protein kinase 1; MAPK 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; protein tyrosine kinase ERK2; mitogen-activated protein kinase 2; extracellular signal-regulated kinase 2
UniProt Protein Name
Mitogen-activated protein kinase 1
UniProt Gene Name
MAPK1
UniProt Synonym Gene Names
ERK2; PRKM1; PRKM2; MAP kinase 1; MAPK 1; ERK-2; p42-MAPK; MAP kinase 2; MAPK 2
UniProt Entry Name
MK01_HUMAN
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Product Notes
The PHOSPHO-Erk 1,2 mapk1 (Catalog #AAA79001) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - PHOSPHO-Erk 1,2 reacts with Human, Mouse Tested: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PHOSPHO-Erk 1,2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PHOSPHO-Erk 1,2 mapk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHOSPHO-Erk 1,2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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