Mouse anti-Human, Mouse PINK1 Monoclonal Antibody | anti-PINK1 antibody

PINK1 Antibody

Cat. Num. AAA290950

• Gene Names: PINK1; BRPK; PARK6
• Reactivity: Human, Mouse
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Synonyms: PINK1, Antibody; PINK1 Antibody; Serine/threonine-protein kinase PINK1; mitochondrial; BRPK; PTEN-induced putative kinase protein 1; PINK1; anti-PINK1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Recombinant PINK1 protein was used to produced this monoclonal antibody.
• Purity/Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 581

• Applicable Applications for anti-PINK1 antibody: ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human colon cancer section using Pink1(Cat#AAA290950). AAA290950 was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H. heart section using Pink1(115-213) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1(115-213) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IF (Immunofluorescence)

(Fluorescent image of PC12 cells stained with Pink1(115-213) Antibody . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

WB (Western Blot)

(All lanes : Anti-Pink1 Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 63 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PINK1 antibody

Background: This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.

References

1. Oxidative stress alters the regulatory control of p66Shc and Akt in PINK1 deficient cells. Maj MC, et al. Biochem Biophys Res Commun, 2010 Aug 27. PMID 20637729. 2. Assessing the prevalence of PINK1 genetic variants in South African patients diagnosed with early- and late-onset Parkinson's disease. Keyser RJ, et al. Biochem Biophys Res Commun, 2010 Jul 16. PMID 20558144. 3. Progression of subtle motor signs in PINK1 mutation carriers with mild dopaminergic deficit. Eggers C, et al. Neurology, 2010 Jun 1. PMID 20513816. 4. Structural imaging in the presymptomatic stage of genetically determined parkinsonism. Reetz K, et al. Neurobiol Dis, 2010 Sep. PMID 20483373. 5. Clinical and demographic characteristics of PINK1 mutation carriers--a meta-analysis. Kasten M, et al. Mov Disord, 2010 May 15. PMID 20461815.

NCBI and Uniprot Product Information

• NCBI GI #: 14165272
• NCBI GeneID: 65018
• NCBI Accession #: NP_115785.1
• NCBI GenBank Nucleotide #: NP_115785.1
• UniProt Accession #: Q9BXM7
• Molecular Weight: H=63;30,M=63;30 KDa
• NCBI Official Full Name: serine/threonine-protein kinase PINK1, mitochondrial
• NCBI Official Synonym Full Names: PTEN induced kinase 1
• NCBI Official Symbol: PINK1
• NCBI Official Synonym Symbols: BRPK; PARK6
• NCBI Protein Information: serine/threonine-protein kinase PINK1, mitochondrial
• UniProt Protein Name: Serine/threonine-protein kinase PINK1, mitochondrial
• UniProt Gene Name: PINK1
• UniProt Entry Name: PINK1_HUMAN