Rabbit PKA R2 Monoclonal Antibody | anti-PRKAR2A antibody

PKA R2 (Phospho-S99) Antibody

Cat. Num. AAA311011

• Gene Names: PRKAR2A; PKR2; PRKAR2
• Reactivity: Human, Mouse, Rat, Pig
• Applications: Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: ProA affinity purified
• Synonyms: PKA R2, Antibody; PKA R2 (Phospho-S99) Antibody; cAMP dependent protein kinase regulatory subunit alpha 2 antibody; cAMP dependent protein kinase regulatory subunit RII alpha antibody; cAMP dependent protein kinase type II alpha regulatory chain antibody; cAMP dependent protein kinase type II alpha regulatory subunit antibody; cAMP-dependent protein kinase type II-alpha regulatory subunit antibody; KAP2 antibody; KAP2_HUMAN antibody; MGC3606 antibody; PKR 2 antibody; PKR2 antibody; PRKA R2 antibody; PRKAR 2 antibody; PRKAR2 antibody; PRKAR2A antibody; Protein kinase A RII alpha subunit antibody; Protein kinase cAMP dependent regulatory type II alpha antibody; anti-PRKAR2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Pig
• Clonality: Monoclonal
• Clone Number: SC54-04
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-PRKAR2A antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser99 of human PKA R2
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

ICC (Immunocytochemistry)

(ICC staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.)

Related Product Information for anti-PRKAR2A antibody

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Calpha, Cbeta and Cgamma, that each represent specific gene products. Calpha and Cbeta are closely related (93% amino acid sequence similarity), whereas Cgamma displays 83% and 79% similarity to Calpha and Cbeta , respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Product Categories/Family for anti-PRKAR2A antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4758958
• NCBI GeneID: 5576
• NCBI Accession #: NP_004148.1
• NCBI GenBank Nucleotide #: NM_004157.2
• UniProt Accession #: P13861; Q16823
• Molecular Weight: 45,518 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type II, alpha
• NCBI Official Symbol: PRKAR2A
• NCBI Official Synonym Symbols: PKR2; PRKAR2
• NCBI Protein Information: cAMP-dependent protein kinase type II-alpha regulatory subunit; protein kinase A, RII-alpha subunit; cAMP-dependent protein kinase regulatory subunit RII alpha
• UniProt Protein Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• UniProt Gene Name: PRKAR2A
• UniProt Synonym Gene Names: PKR2; PRKAR2
• UniProt Entry Name: KAP2_HUMAN