Mouse PKCdelta Monoclonal Antibody | anti-PKCdelta antibody

PKCdelta (N-terminal region) Antibody

Cat. Num. AAA71685

• Gene Names: PRKCD; MAY1; PKCD; nPKC-delta
• Reactivity: Human, Rat, Mouse
• Applications: Immunocytochemistry, ELISA, Western Blot
• Purity: Protein A chromatography
• Synonyms: PKCdelta, Antibody; PKCdelta (N-terminal region) Antibody; PKCdelta (N-terminal region); anti-PKCdelta antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: M242
• Specificity: This antibody detects a 78kDa* protein corresponding to the molecular mass of PKCdelta on SDS-PAGE immunoblots of adult mouse brain tissue lysate.
• Purity/Purification: Protein A chromatography
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-PKCdelta antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: Clone (M242) was generated from a recombinant human protein that included amino acids residues in the N-terminal region. This sequence is conserved in rat and mouse PKCdelta, and has low homology to other PKC family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of PKCdelta in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKCdelta (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.)

WB (Western Blot)

(Western blot analysis of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-PKCdelta (N-terminal region) at 1:125 (lane 1) and 1:500 (lane 2).)

Related Product Information for anti-PKCdelta antibody

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (alpha, beta1, betaII, and gamma) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity.

References

Kawakami et al. (2003) PNAS. USA 100:9470-9475.
Nishizuka, Y. (1988) Nature 334:661.

NCBI and Uniprot Product Information

• NCBI GI #: 31377782
• NCBI GeneID: 5580
• NCBI Accession #: NP_006245.2
• NCBI GenBank Nucleotide #: NM_006254.3
• UniProt Accession #: Q05655; Q15144; Q86XJ6; B0KZ81; B2R834
• Molecular Weight: 77,505 Da
• NCBI Official Full Name: protein kinase C delta type
• NCBI Official Synonym Full Names: protein kinase C, delta
• NCBI Official Symbol: PRKCD
• NCBI Official Synonym Symbols: MAY1; PKCD; nPKC-delta
• NCBI Protein Information: protein kinase C delta type; protein kinase C delta VIII; tyrosine-protein kinase PRKCD
• UniProt Protein Name: Protein kinase C delta type
• UniProt Gene Name: PRKCD
• UniProt Synonym Gene Names: SDK1
• UniProt Entry Name: KPCD_HUMAN