IP (Immunoprecipitation)
(Immunoprecipitation analysis of U87 cell lysates using PKM2 antibody. IP:PKM2 Monoclonal Antibody. WB:PKM2 Polyclonal Antibody #21578.)
Mouse anti-Human PKM2 Monoclonal Antibody | anti-PKM2 antibody
PKM2 Antibody
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
PKM2, Antibody; PKM2 Antibody; PKM; PK3; OIP3; PK2; anti-PKM2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
XD2014M001S1
Specificity
The antibody detects endogenous levels of total PKM2 protein
Form/Format
1.0 mg/mL in phosphate buffer without Mg2+/Ca2+, pH7.4, 150mM NaCl and 50% glycerol
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
605
Applicable Applications for anti-PKM2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant human PKM2 protein
Target Name
PKM2
Preparation and Storage
Store at -20 degree C
IP (Immunoprecipitation)
(Immunoprecipitation analysis of U87 cell lysates using PKM2 antibody. IP:PKM2 Monoclonal Antibody. WB:PKM2 Polyclonal Antibody #21578.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of U87 cell lysates using PKM2 antibody. IP:PKM2 Monoclonal Antibody. WB:PKM2 Polyclonal Antibody #21578.)
WB (Western Blot)
(Western blot analysis of extracts from U87 cells using PKM2 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from U87 cells using PKM2 Antibody.)
Related Product Information for anti-PKM2 antibody
Pyruvate kinase (PK) regulates the final rate-limiting step of glycolysis in the production of pyruvate and adenosine triphosphate (ATP). Alternate splicing of PKM pre-mRNA leads to PKM2 generation by the inclusion of exon 10 and the exclusion of exon 9, which is specific for PKM1. Besides its cytosolic roles in glycolysis, PKM2, which is upregulated by growth factor receptor activation 1, is phosphorylated at S37 by extracellular signal-regulated kinase (ERK) 2. This phosphorylation leads to the cis-trans isomerization of PKM2 by the peptidyl-prolineisomerase protein interacting with never in mitosis A 1 (PIN1), exposure of the nuclear localization signal (NLS) of PKM2, and subsequent binding of importin α 5 for nuclear translocation 2. In the nucleus, PKM2binds to phosphorylated Y333 of b-catenin, which is essential for b-catenin transactivation 3, and interacts with and phosphorylates histone H3 at T11, leading to H3-K9 acetylation and transcription of genes such as MYC and CCND14. c-Myc expression results in the upregulation of GLUT1, lactate dehydrogenase A (LDHA), and, in a positive feedback loop, PTB-dependent PKM2, which subsequently enhances the Warburg effect 2. Cyclin D1 expression, in turn, promotes G1-S phase transition 3, 4.
Product Categories/Family for anti-PKM2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,273 Da
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
NCBI Protein Information
pyruvate kinase PKM
UniProt Protein Name
Pyruvate kinase PKM
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN
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Product Notes
The PKM2 pkm (Catalog #AAA300720) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PKM2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKM2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PKM2 pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKM2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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