Mouse PlGF-2 Monoclonal Antibody | anti-PlGF-2 antibody

Mouse Anti-Human PlGF-2

Cat. Num. AAA79330

• Reactivity: No cross reactivity with mouse and rat PIGF.
• Applications: Immunofluorescence, Western Blot, ELISA
• Purity: Protein G purified
• Synonyms: PlGF-2, Antibody; Mouse Anti-Human PlGF-2; PlGF; placental growth factor; anti-PlGF-2 antibody

• Host: Mouse
• Reactivity: No cross reactivity with mouse and rat PIGF.
• Clonality: Monoclonal
• Isotype: IgG₁
• Clone Number: 3B3/A9/A6/D1
• Purity/Purification: Protein G purified
• Form/Format: Sterile filtered antibody solution was lyophilized from PBS; does not contain stabilizers or preservatives.

• Applicable Applications for anti-PlGF-2 antibody: IF (Immunofluorescence), WB (Western Blot), ELISA
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Preparation: Monoclonal antibodies were produced with the help of BALB/c mice using recombinant human PlGF-2 [Leu19 - Arg170] as the immunizing antigen.
• Immunogen: Recombinant human PlGF-2(Cat#MBS691507)
• Preparation and Storage:

  Product Form	Temperature	Storage Time
  Lyophilized	-20°C to -80°C	Long Term
  Lyophilized	4°C	3 Months
  Lyophilized	Room Temperature	1 Month
  Reconstituted	2°C to 8°C	2 Weeks
  Extended Storage	-20°C to -80°C	6 Months

  ; Extended Storage ; After initial reconstitution, the addition of a carrier protein or 50% glycerol is recommended for extended storage. Prepare and store working aliquots at -20°C to -80°C.

IF (Immunofluorescence)

(IF staining of human PlGF1 and PlGF2 in Sf9 cells expressing either PlGF1 or PlGF2. Upper lane: mouse anti-human PlGF2 #D1 [Cat# AAA79330] recognizing only PlGF2 and mouse anti-human PlGF #178/G10 [Cat# recognizing both isoforms of PlGF.)

Application Data

(Human sFlt1 (Cat# was coated with 1ug/ml to a 96-well plate. Recombinant human PlGF2 was pre-incubated with a 10x and 50x excess of anti-human PlGF2 (Cat# AAA79330, recognizing only PlGF2) and anti-human PlGF (Cat# recognizing both isoforms) for 90min. Then PlGF2 alone and the approaches with the antibodies were added. Pre- incubation of the PlGF2-specific antibody #A6D1 with PlGF2 did not block the binding of PlGF2 to its receptor sFlt1. In contrast, pre-incubation of PlGF2 with Direct ELISA with recombinant human PlGF1 and PlGF2 derived from insect cells. Both proteins were coated to a 96-well plate [0.2ug/ml] and anti-human PlGF #331/H12 [Cat# recognizing both isoforms and the anti-human PlGF2 #D1 [Cat# AAA79330] recognizing specifically PlGF2 were added with increasing concentrations. Detection was carried out with an anti-mouse Biotin- conjugated antibody (Dianova))

SDS-PAGE

(Purified recombinant human PlGF2 (100 ng/lane) derived from insect cells was loaded in 12.5% SDS-PAGE under reducing conditions. As primary antibody mouse anti-human PlGF2 #D1 was used in a concentration of 1ug/ml (A), 0.5ug/ml (B), 0.1ug/ml (C) and 0.05ug/ml (D). The detection was carried out with a secondary, AP-conjugated anti-mouse Fc antibody. NOTE: There is still a signal visible for PlGF2 at an antibody concentration of 50ng/ml.)

SDS-PAGE

(Purified recombinant human PlGF2 (500 -10 ng/lane), PlGF1 (1000 ng/lane), and mouse and rat PlGF (500ng/lane) all derived from insect cells were loaded in 12.5% SDS-PAGE under reducing conditions. As primary antibody mouse anti-human PlGF2 #D1 was used in a concentration of 2ug/ml. The detection was carried out with a secondary, AP-conjugated anti- mouse Fc antibody. There is no cross reaction with human PlGF1, mouse and rat PlGF)

WB (Western Blot)

(Western analysis with human PlGF1 and PlGF2. Purified recombinant human PlGF1 (red: 500ng, non-red: 1000ng) and PlGF2 (red: 250ng, non-red: 500ng) were loaded in 15% SDS-PAGE under reducing and non-reducing conditions. As primary antibody mouse anti-human PlGF2 #D1 was used in a concentration of 0.5ug/ml. The detection was carried out with a secondary, AP-conjugated anti-mouse Fc antibody (Dianova, 1:1000). The anti-human PlGF-2 #D1 recognizes solely the PlGF2 isoform.)

Related Product Information for anti-PlGF-2 antibody

Human Placenta Growth Factor-2 (PlGF-2), a 22 kDa protein consisting of 152 amino acid residues is produced as a homodimer. PlGF is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF acts only as a weak mitogen for those cell types possessing receptors for binding (e.g. vascular endothelial cells). At least one high-affinity receptor for PlGF (FLT-1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human umbilical vein endothelial cells and monocytes). In addition to its action as a weak mitogen it is also a chemoattractant for monocytes and endothelial cells. Three different proteins are generated by differential splicing of the human PlGF gene: PlGF-1 (131aa native chain), PlGF-2 (152aa) and PlGF-3 (203aa). All 3 mitogens are secretable proteins, but PlGF-2 can bind to heparin with high affinity. PlGF is apparently a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms possess similar biological activities. Heterodimers between VEGF and PlGF are mitogenic for endothelial cells and have strong angiogenic activity in vivo (e.g. in the CAM assay or in the cornea pocket assay). Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A much related protein of PlGF is VEGF with about 53% homology and VEGF-B with similar biological activities.

NCBI and Uniprot Product Information

• NCBI Accession #: NP_001193941.1
• NCBI GenBank Nucleotide #: NM_001207012.1
• UniProt Accession #: P49763
• Molecular Weight: ~50 kDa HC and ~25 kDa LC