Mouse anti-Human PlGF-2 Monoclonal Antibody | anti-PlGF-2 antibody

Mouse Anti-Human PlGF-2

Cat. Num. AAA79093

• Gene Names: PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Protein G purified
• Synonyms: PlGF-2, Antibody; Mouse Anti-Human PlGF-2; Anti-human PlGF-2 (#3B10); Placenta growth factor; PlGF; anti-PlGF-2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: IgG1; 3B10
• Purity/Purification: Protein G purified
• Form/Format: Lyophilized
• Sequence Length: 149

• Applicable Applications for anti-PlGF-2 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Recombinant human PlGF-2 (RT #300-019)
• Buffer: PBS
• Preparation and Storage: The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0 degree C. Reconstituted anti-PlGF-2 is stable at 4 degree C for > 6 month or can be stored in working aliquots at -20 degree C for more than six months.

Application Data

Application Data

Related Product Information for anti-PlGF-2 antibody

Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF1), 152 (PlGF2), and 203 (PlGF3) amino acids (aa) respectively. Only PlGF2 contains a highly basic heparinbinding 21 aa insert at the C-terminus. In the mouse, only one P lGF that is the equivalent of human PlGF2 has been identified. Human PlGF1 shares 56%, 55%, 74% and 95% aa identity with the appropriate isoform of mouse, rat, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2mediated angiogenesis. However, PlGF (especially PlGF1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF2, but not PLGF-1, shows heparindependent binding of neuropilin (Npn)-1 and Npn2. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis.

References

1. DiPalma, T. et al. (1996) Mamm. Genome 7:6. 2. Cao, Y. et al. (1997) Biochem. Biophys. Res. Commun. 235:493. 3. Ferrara, N. et al. (1997) Endocrin. Rev. 18:4 4. Kim KJ et al, Exp Mol Med 44:10-9, 2012 5. De Falco S, Exp Mol Med 44:1-9, 2012

NCBI and Uniprot Product Information

• NCBI GI #: 333033785
• NCBI GeneID: 5228
• NCBI Accession #: NP_001193941.1
• NCBI GenBank Nucleotide #: NM_001207012.1
• UniProt Accession #: P49763; Q07101; Q9BV78; Q9Y6S8
• Molecular Weight: 24,789 Da
• NCBI Official Full Name: placenta growth factor isoform 2
• NCBI Official Synonym Full Names: placental growth factor
• NCBI Official Symbol: PGF
• NCBI Official Synonym Symbols: PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
• NCBI Protein Information: placenta growth factor; placental growth factor, vascular endothelial growth factor-related protein
• UniProt Protein Name: Placenta growth factor
• UniProt Gene Name: PGF
• UniProt Synonym Gene Names: PGFL; PLGF; PlGF
• UniProt Entry Name: PLGF_HUMAN