WB (Western Blot)
(PODXL Antibody (C-term)(Ascites) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PODXL antibody detected the PODXL protein (arrow).)
Mouse anti-Human PODXL Monoclonal Antibody | anti-PODXL antibody
PODXL Antibody (C-term) (Ascites)
Gene Names
PODXL; PC; PCLP; Gp200; PCLP-1
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence
Synonyms
PODXL, Antibody; PODXL Antibody (C-term) (Ascites); Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1; PODXL; PCLP; PCLP1; anti-PODXL antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
632CT5.11.4
Specificity
This PODXL antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL.
Form/Format
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Sequence Positions
410-439
Sequence Length
558
Applicable Applications for anti-PODXL antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(PODXL Antibody (C-term)(Ascites) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PODXL antibody detected the PODXL protein (arrow).)
WB (Western Blot)
(PODXL Antibody (C-term)(Ascites) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PODXL antibody detected the PODXL protein (arrow).)
IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with PODXL (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA283585 PODXL (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).)
IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with PODXL (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA283585 PODXL (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).)
Related Product Information for anti-PODXL antibody
This gene encodes a member of the sialomucin protein
family. The encoded protein was originally identified as an
important component of glomerular podocytes. Podocytes are highly
differentiated epithelial cells with interdigitating foot processes
covering the outer aspect of the glomerular basement membrane.
Other biological activities of the encoded protein include: binding
in a membrane protein complex with Na+/H+ exchanger regulatory
factor to intracellular cytoskeletal elements, playing a role in
hematopoetic cell differentiation, and being expressed in vascular
endothelium cells and binding to L-selectin.
family. The encoded protein was originally identified as an
important component of glomerular podocytes. Podocytes are highly
differentiated epithelial cells with interdigitating foot processes
covering the outer aspect of the glomerular basement membrane.
Other biological activities of the encoded protein include: binding
in a membrane protein complex with Na+/H+ exchanger regulatory
factor to intracellular cytoskeletal elements, playing a role in
hematopoetic cell differentiation, and being expressed in vascular
endothelium cells and binding to L-selectin.
Product Categories/Family for anti-PODXL antibody
References
References for protein:
1.Schopperle, W.M., et al. Biochem. Biophys. Res. Commun. 398(3):372-376(2010)
2.Hsu, Y.H., et al. Am. J. Pathol. 176(6):3050-3061(2010)
3.Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010)
4.Drossopoulou, G.I., et al. Am. J. Physiol. Renal Physiol. 297 (3), F594-F603 (2009)
5.Thomas, S.N., et al. Am. J. Physiol., Cell Physiol. 296 (3), C505-C513 (2009)
References for SY5Y (SH-SY5Y; #CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
1.Schopperle, W.M., et al. Biochem. Biophys. Res. Commun. 398(3):372-376(2010)
2.Hsu, Y.H., et al. Am. J. Pathol. 176(6):3050-3061(2010)
3.Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010)
4.Drossopoulou, G.I., et al. Am. J. Physiol. Renal Physiol. 297 (3), F594-F603 (2009)
5.Thomas, S.N., et al. Am. J. Physiol., Cell Physiol. 296 (3), C505-C513 (2009)
References for SY5Y (SH-SY5Y; #CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58635
NCBI Official Full Name
podocalyxin isoform 1
NCBI Official Synonym Full Names
podocalyxin-like
NCBI Official Symbol
PODXL
NCBI Official Synonym Symbols
PC; PCLP; Gp200; PCLP-1
NCBI Protein Information
podocalyxin
UniProt Protein Name
Podocalyxin
UniProt Gene Name
PODXL
UniProt Synonym Gene Names
PCLP; PCLP1; PC; PCLP-1
UniProt Entry Name
PODXL_HUMAN
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Product Notes
The PODXL podxl (Catalog #AAA283585) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 410-439. The PODXL Antibody (C-term) (Ascites) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PODXL can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the PODXL podxl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PODXL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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