Mouse anti-Human PP2C alpha/PPM1A Monoclonal Antibody | anti-PPM1A antibody

PP2C alpha/PPM1A

Cat. Num. AAA48287

• Gene Names: PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: Protein-A Affinity Chromatography
• Synonyms: PP2C alpha/PPM1A, Antibody; PP2C alpha/PPM1A; Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform; PDP1; PDP; PPM2C; PP2C alpha; Protein phosphatase 2C; PDP 1; EC 3.1.3.43; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1; Pyruvate dehydrogenase acetyl-transferring-phosphatase 1; PPM1A; Protein phosphatase 1A (formerly 2C); ppm1a; FLJ42306; MGC9201; PP2CA; PP2Calpha; PP2C-alpha; protein phosphatase Mg2+/Mn2+ dependent 1A; anti-PPM1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b kappa
• Clone Number: p6C7
• Purity/Purification: Protein-A Affinity Chromatography
• Form/Format: Liquid; In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 455

• Applicable Applications for anti-PPM1A antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant human PP2Calpha/PPM1A (1-382aa) purified from E Coli.
• Conjugation: Unconjugated
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

WB (Western Blot)

(The cell lysates (40ug) and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha/PPM1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1: Jurkat cell lysate;Lane 2: HeLa cell lysate;Lane 3: K-562 cell lysate;Lane 4: MCF7 cell lysate;Lane 5: A549 cell lysate;Lane 6: Raji cell lysate;Lane 7: Mouse kidney tissue lysate;Lane 8: Mouse brain tissue lysate;Lane 9: Mouse liver tissue lysate)

ICC (Immunocytochemistry)

(ICC/IF analysis of PP2C alpha/PPM1A in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Related Product Information for anti-PPM1A antibody

Protein phosphatase 2C (PP2Calpha) is a Mn2+-or Mg 2+ -dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNk MAPk pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signaling kinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2Calpha, the prototypical member of the PPM family.

Product Categories/Family for anti-PPM1A antibody

Cancer; Epigenetics and Nuclear Signaling; Metabolism; Signal Transduction

References

Lin X, et al., (2006) Cell. 125(5): 915-928. Duan X, et al., (2006) J Biol Chem. 281(48):36526-32.

NCBI and Uniprot Product Information

• NCBI GI #: 193211600
• NCBI GeneID: 5494
• NCBI Accession #: NP_808821.2
• NCBI GenBank Nucleotide #: NP_808821.2
• UniProt Accession #: P35813
• NCBI Official Full Name: protein phosphatase 1A isoform 3
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent 1A
• NCBI Official Symbol: PPM1A
• NCBI Official Synonym Symbols: PP2CA; PP2Calpha; PP2C-ALPHA
• NCBI Protein Information: protein phosphatase 1A
• UniProt Protein Name: Protein phosphatase 1A
• UniProt Gene Name: PPM1A
• UniProt Synonym Gene Names: PPPM1A; PP2C-alpha
• UniProt Entry Name: PPM1A_HUMAN