WB (Western Blot)
(Figure 1. Western blot analysis of PPM1A using anti-PPM1A antibody.)
Rabbit anti-Human PPM1A Polyclonal Antibody | anti-PPM1A antibody
Anti-PPM1A Antibody
Gene Names
PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
PPM1A, Antibody; Anti-PPM1A Antibody; Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA; PPM1A; PPPM1A; anti-PPM1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
455
Applicable Applications for anti-PPM1A antibody
ELISA (Direct ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence of human PPM1A(HAGSQVAKYCCEHLLDHITNNQDFK).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of PPM1A using anti-PPM1A antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of PPM1A using anti-PPM1A antibody.)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-PPM1A antibody
Description: Rabbit IgG polyclonal antibody for PPM1A detection. Tested with WB, IHC-P, Direct ELISA in Human.
Background: Protein phosphatase 1A is an enzyme that in humans is encoded by the PPM1A gene. It is mapped to 14q23.1. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Background: Protein phosphatase 1A is an enzyme that in humans is encoded by the PPM1A gene. It is mapped to 14q23.1. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
References
1. Lin, X., Duan, X., Liang, Y.-Y., Su, Y., Wrighton, K. H., Long, J., Hu, M., Davis, C. M., Wang, J., Brunicardi, F. C., Shi, Y., Chen, Y.-G., Meng, A., Feng, X.-H. PPM1A functions as a Smad phosphatase to terminate TGF-beta signaling. Cell 125: 915-928, 2006. Note: Editorial Expression of Concern: Cell 165: 498 only, 2016. Editoral Note: Cell 166: 1597 only, 2016.
2. Mann, D. J., Campbell, D. G., McGowan, C. H., Cohen, P. T. W. Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim. Biophys. Acta 1130: 100-104, 1992.
3. Takekawa, M., Maeda, T., Saito, H. Protein phosphatase 2C-alpha inhibits the human stress-responsive p38 and JNK MAPK pathways. EMBO J. 17: 4744-4752, 1998.
2. Mann, D. J., Campbell, D. G., McGowan, C. H., Cohen, P. T. W. Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim. Biophys. Acta 1130: 100-104, 1992.
3. Takekawa, M., Maeda, T., Saito, H. Protein phosphatase 2C-alpha inhibits the human stress-responsive p38 and JNK MAPK pathways. EMBO J. 17: 4744-4752, 1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein phosphatase 1A isoform 3
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1A
NCBI Official Symbol
PPM1A
NCBI Official Synonym Symbols
PP2CA; PP2Calpha; PP2C-ALPHA
NCBI Protein Information
protein phosphatase 1A
UniProt Protein Name
Protein phosphatase 1A
UniProt Gene Name
PPM1A
UniProt Synonym Gene Names
PPPM1A; PP2C-alpha
UniProt Entry Name
PPM1A_HUMAN
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Product Notes
The PPM1A ppm1a (Catalog #AAA125457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPM1A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1A can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PPM1A ppm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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