WB (Western Blot)
(Western BlotanalysisofHu,Ms,RtKidneyCells(HEK293),AlveolarBasalEpithelialcells(A549)showingdetectionof~55PPM1HproteinusingMouseAnti-PPM1HMonoclonalAntibody,Clone10D2 .Lane1:MWLadder.Lane2:A549.Lane3:HEK293..Load:30ug.Block:5%SkimMilkinTBST,1h,RT.PrimaryAntibody:MouseAnti-PPM1HMonoclonalAntibody at1:1000forOvernight,4C.SecondaryAntibody:Donkeyanti-mouse800(LiCOR)at1:25000for1houratRT.ColorDevelopment:OdysseyCLxWestern Blotimaging.Predicted/ObservedSize:~55.OtherBand(s):~30,~100.Courtesyof:DarioAlessiLab,UniversityofDundee.)
Mouse PPM1H IgG1 Monoclonal Antibody | anti-PPM1H antibody
Human Anti-Human PPM1H Monoclonal IgG1
Gene Names
PPM1H; URCC2; ARHCL1; NERPP-2C
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein G Purified
Synonyms
PPM1H IgG1, Antibody; Human Anti-Human PPM1H Monoclonal IgG1; PPM1H Antibody, Clone 10D2: HRP; PPM1H Antibody: HRP; PPM1H; Protein phosphatase Mg2+/Mn2+ dependent 1H Antibody; NERPP2C; Protein phosphatase 1H Antibody; anti-PPM1H antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10D2
Specificity
Detects ~55kDa
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PPM1H antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen Species
Human
Immunogen
Recombinant Human PPM1H Protein
Conjugation
HRP
Tissue Specificity
Parathyroid gland
Research Area
Neuroscience, Neurodegeneration, Parkinson's Disease, Cancer, Tumor Biomarkers, Oncoproteins, Signal Transducers, Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases
Cellular Localization
Intracellular
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of PPM1H in 10 ug of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western BlotanalysisofHu,Ms,RtKidneyCells(HEK293),AlveolarBasalEpithelialcells(A549)showingdetectionof~55PPM1HproteinusingMouseAnti-PPM1HMonoclonalAntibody,Clone10D2 .Lane1:MWLadder.Lane2:A549.Lane3:HEK293..Load:30ug.Block:5%SkimMilkinTBST,1h,RT.PrimaryAntibody:MouseAnti-PPM1HMonoclonalAntibody at1:1000forOvernight,4C.SecondaryAntibody:Donkeyanti-mouse800(LiCOR)at1:25000for1houratRT.ColorDevelopment:OdysseyCLxWestern Blotimaging.Predicted/ObservedSize:~55.OtherBand(s):~30,~100.Courtesyof:DarioAlessiLab,UniversityofDundee.)
WB (Western Blot)
(Western BlotanalysisofHu,Ms,RtKidneyCells(HEK293),AlveolarBasalEpithelialcells(A549)showingdetectionof~55PPM1HproteinusingMouseAnti-PPM1HMonoclonalAntibody,Clone10D2 .Lane1:MWLadder.Lane2:A549.Lane3:HEK293..Load:30ug.Block:5%SkimMilkinTBST,1h,RT.PrimaryAntibody:MouseAnti-PPM1HMonoclonalAntibody at1:1000forOvernight,4C.SecondaryAntibody:Donkeyanti-mouse800(LiCOR)at1:25000for1houratRT.ColorDevelopment:OdysseyCLxWestern Blotimaging.Predicted/ObservedSize:~55.OtherBand(s):~30,~100.Courtesyof:DarioAlessiLab,UniversityofDundee.)
ICC (Immunocytochemistry)
(Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-PPM1HMonoclonalAntibody,Clone10D2 .Tissue:AlveolarBasalEpithelialcells(A549).Species:Hu,Ms,Rt.PrimaryAntibody:MouseAnti-PPM1HMonoclonalAntibody at1:1000for1houratRT.SecondaryAntibody:AlexaFluor594Donkeyantimousesecondarydilutedin0.2%BSA+0.02%sodiumazideinPBSat1:1000for1houratRT.Counterstain:Cellsmountedandco-stainedwithDAPIusingProLongGoldantifadereagentwithDAPI(Invitrogen).Localization:Intracellular,Nucleoplasm.Magnification:63x.Courtesyof:DarioAlessiLab,UniversityofDundee.)
ICC (Immunocytochemistry)
(Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-PPM1HMonoclonalAntibody,Clone10D2 .Tissue:AlveolarBasalEpithelialcells(A549).Species:Hu,Ms,Rt.PrimaryAntibody:MouseAnti-PPM1HMonoclonalAntibody at1:1000for1houratRT.SecondaryAntibody:AlexaFluor594Donkeyantimousesecondarydilutedin0.2%BSA+0.02%sodiumazideinPBSat1:1000for1houratRT.Counterstain:Cellsmountedandco-stainedwithDAPIusingProLongGoldantifadereagentwithDAPI(Invitrogen).Localization:Intracellular,Nucleoplasm.Magnification:63x.Courtesyof:DarioAlessiLab,UniversityofDundee.)
Related Product Information for anti-PPM1H antibody
PPM1H is an independent prognostic biomarker of non-small cell lung cancer (1). PPM1H acts as a key modulator of LRRK2 signaling by controlling dephosphorylation of Rab proteins (2). PPM1H is also suggested to be a gatekeeper to prevent excessive bone morphogenetic protein (BMP) signalling through dephosphorylation (3).
Product Categories/Family for anti-PPM1H antibody
References
1. Zhang W.Q., et al. (2021) Neoplasma. 68(5): 917-923. 2. Berndsen K., et al. (2019) 8:e50416. 3.Shen T., et al. (2014) Cell Res. 24(6): 727-741.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,448 Da
NCBI Official Full Name
protein phosphatase 1H
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent, 1H
NCBI Official Symbol
PPM1H
NCBI Official Synonym Symbols
URCC2; ARHCL1; NERPP-2C
NCBI Protein Information
protein phosphatase 1H; ras homolog gene family, member C like 1; protein phosphatase 1H (PP2C domain containing); neurite extension-related protein phosphatase related to PP2C
UniProt Protein Name
Protein phosphatase 1H
UniProt Gene Name
PPM1H
UniProt Synonym Gene Names
ARHCL1; KIAA1157; URCC2
UniProt Entry Name
PPM1H_HUMAN
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Product Notes
The PPM1H ppm1h (Catalog #AAA254077) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Human Anti-Human PPM1H Monoclonal IgG1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1H IgG1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PPM1H ppm1h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1H IgG1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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