WB (Western Blot)
(All lanes : Anti-PRDM16 Antibody at 1:4000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 140 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse anti-Human PRDM16 Monoclonal Antibody | anti-PRDM16 antibody
PRDM16 Antibody
Gene Names
PRDM16; MEL1; LVNC8; PFM13; CMD1LL
Reactivity
Human
Applications
ELISA, Western Blot
Synonyms
PRDM16, Antibody; PRDM16 Antibody; PR domain zinc finger protein 16; PR domain-containing protein 16; Transcription factor MEL1; MDS1/EVI1-like gene 1; PRDM16; KIAA1675; MEL1; PFM13; Purified Mouse Monoclonal Antibody (Mab); anti-PRDM16 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
This PRDM16 antibody is generated from a mouse immunized with a recombinant protein between 779-996 amino acids from PRDM16.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
1276
Applicable Applications for anti-PRDM16 antibody
ELISA, WB (Western Blot)
Function
Binds DNA and functions as a transcriptional regulator (PubMed: ). Displayshistone methyltransferase activity andmonomethylates 'Lys-9' of histone H3(H3K9me1) in vitro (By similarity). Probablycatalyzes the monomethylation of freehistone H3 in the cytoplasm which is thentransported to the nucleus and incorporated into nucleosomes where SUV39 Hmethyltransferases use it as a substrate tocatalyze histone H3 'Lys-9' trimethylation (By similarity). Likely to be one of the primaryhistone methyltransferases along withMECOM/PRDM3 that direct cytoplasmicH3K9me1 methylation (By similarity).Functions in the differentiation of brownadipose tissue (BAT) which is specialized in dissipating chemical energy in the form ofheat in response to cold or excess feedingwhile white adipose tissue (WAT) isspecialized in the storage of excess energyand the control of systemic metabolism (By similarity). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (By similarity). Functions as a repressor of TGF-betasignaling (PubMed: ).
Cellular Location
Nucleus.
Tissue Location
Expressed in uterus and kidney. Expressed in both cardiomyocytes and interstitial cells
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-PRDM16 Antibody at 1:4000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 140 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PRDM16 Antibody at 1:4000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 140 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PRDM16 antibody
Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.
Product Categories/Family for anti-PRDM16 antibody
References
Mochizuki N.,et al.Blood 96:3209-3214(2000). Fang W.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases. Nagase T.,et al.DNA Res. 7:347-355(2000). Nakajima D.,et al.DNA Res. 9:99-106(2002). Gregory S.G.,et al.Nature 441:315-321(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
PR domain zinc finger protein 16
NCBI Official Synonym Full Names
PR/SET domain 16
NCBI Official Symbol
PRDM16
NCBI Official Synonym Symbols
MEL1; LVNC8; PFM13; CMD1LL
NCBI Protein Information
PR domain zinc finger protein 16
UniProt Protein Name
PR domain zinc finger protein 16
UniProt Gene Name
PRDM16
UniProt Synonym Gene Names
MDS1/EVI1-like gene 1
UniProt Entry Name
PRD16_HUMAN
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Product Notes
The PRDM16 prdm16 (Catalog #AAA290741) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRDM16 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRDM16 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PRDM16 prdm16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDM16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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