SDS-PAGE
(SDS-PAGE Analysis Purified Prohibitin Mouse Monoclonal Antibody (SPM311). Confirmation of Purity and Integrity of Antibody.)
Mouse anti-Human Prohibitin Monoclonal Antibody | anti-PHB antibody
Prohibitin (Mitochondrial Marker)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Prohibitin, Antibody; Prohibitin (Mitochondrial Marker); Epididymis luminal protein 215; Epididymis secretory sperm binding protein Li 54e; HEL 215; HEL S 54e; PHB; PHB1; Prohibitin; anti-PHB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2c, kappa
Clone Number
SPM311
Specificity
Recognizes a protein of 30kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
272
Applicable Applications for anti-PHB antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Positive Control
HepG2, A431 or MCF-7 cells. Liver, Kidney, Heart or Endometrium.
Immunogen
Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)
Cellular Localization
Cytoplasmic (Mitochondria)
Hu-chromosome Location
17q21.33
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis Purified Prohibitin Mouse Monoclonal Antibody (SPM311). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified Prohibitin Mouse Monoclonal Antibody (SPM311). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Hepatic Carcinoma stained with Prohibitin Mouse Monoclonal Antibody (SPM311).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Hepatic Carcinoma stained with Prohibitin Mouse Monoclonal Antibody (SPM311).)
References
McClung, J.K., Jupe, E.R., Liu, X-T., and Dell'Orco, R.T. Prohibitin: Potential role in senescence, development, and tumor suppression. Exp. Gerontol. 1995, 30(2):99-124.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
30kDa
NCBI Official Full Name
prohibitin
NCBI Official Synonym Full Names
prohibitin
NCBI Official Symbol
PHB
NCBI Official Synonym Symbols
PHB1; HEL-215; HEL-S-54e
NCBI Protein Information
prohibitin
UniProt Protein Name
Prohibitin
UniProt Gene Name
PHB
UniProt Entry Name
PHB_HUMAN
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Product Notes
The PHB phb (Catalog #AAA215140) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Prohibitin (Mitochondrial Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Prohibitin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PHB phb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prohibitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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