Rabbit anti-Human PHB/Prohibitin Polyclonal Antibody | anti-PHB antibody

Anti-PHB/Prohibitin Antibody, Rabbit Polyclonal

Cat. Num. AAA256111

• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PHB/Prohibitin, Antibody; Anti-PHB/Prohibitin Antibody, Rabbit Polyclonal; PHB/Prohibitin Antibody, Rabbit PAb, Antigen Affinity Purified; prohibitin; Anti-HEL-215 Antibody; Anti-HEL-S-54e Antibody; Anti-PHB1 Antibody; anti-PHB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PHB/Prohibitin
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PHB antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human PHB/Prohibitin fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PHB/Prohibitin fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(PHB was immunoprecipitated using:Lane A:0.5 mg HEK-293 Whole Cell Lysate4 uL anti-PHB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PHB rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size :27 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of PHB in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PHB polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human PHB in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-PHB rabbit polyclonal antibody at 1:500 dilutionLane A: HEK-293 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: U-87 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDa)

Related Product Information for anti-PHB antibody

Prohibitin (PHB) is overtly conserved evolutionarily and ubiquitously expressed protein with pleiotropic functions in diverse cellular compartments. PHB is a ubiquitous protein that is mainly localized in the inner mitochondrial membrane and exerts various mitochondrial functions. PHB may be a substrate of poly-ubiquitin to regulate the ubiquitination of mitochondria and even subsequent elimination during P. esculenta spermiogenesis, and it has a potential role in guaranteeing the maternal inheritance of mitochondria. PHB might play important roles during spermatogenesis and oogenesis by regulating mitochondrial activities. There are several studies reported to understand and decipher PHB's role in diseases and/or cancers of ovary, lung, stomach, thyroid, liver, blood, prostate, gastric, esophagus, glioma, breast, bladder, etc. where PHB is shown to act through mechanisms by acting as an oncogene, tumor suppressor, antioxidant, antiapoptotic, in angiogenesis, autophagy, etc.

References

Qiu X, et al. (2020) Characterization of phb in the gonadal development of the swimming crab portunus trituberculatus. Comp Biochem Physiol B Biochem Mol Biol 240 110338.Hou CC, et al. (2018) The expression pattern and potential functions of phb in the spermiogenesis of phascolosoma esculenta. Gene 652 25-38.Chowdhury D, et al. (2017) Phb in cardiovascular and other diseases: Present knowledge and implications. Curr Drug Targets 18 (16): 1836-1851.