WB (Western Blot)
(Western blot analysis of extracts from various samples, using Prohibitin Mouse Monoclonal Ab.Lane 1: (CAT# please inquire) cells treated with blocking-peptides,Lane 2: (CAT# please inquire) cells,Lane 3: Mouse heart tissue,Lane 4: Rat heart tissue.)
Mouse Prohibitin Monoclonal Antibody | anti-PHB antibody
Prohibitin Mouse Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-chromatography.
Synonyms
Prohibitin, Antibody; Prohibitin Mouse Monoclonal Antibody; Epididymis luminal protein 215; Epididymis secretory sperm binding protein Li 54e; HEL 215; HEL S 54e; PHB; PHB_HUMAN; PHB1; Prohibitin; anti-PHB antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Specificity
Prohibitin Antibody detects endogenous levels of total Prohibitin.
Purity/Purification
Affinity-chromatography.
Form/Format
Supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/mL (varies by lot)
Applicable Applications for anti-PHB antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human Prohibitin, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Prohibitin Mouse Monoclonal Ab.Lane 1: (CAT# please inquire) cells treated with blocking-peptides,Lane 2: (CAT# please inquire) cells,Lane 3: Mouse heart tissue,Lane 4: Rat heart tissue.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Prohibitin Mouse Monoclonal Ab.Lane 1: (CAT# please inquire) cells treated with blocking-peptides,Lane 2: (CAT# please inquire) cells,Lane 3: Mouse heart tissue,Lane 4: Rat heart tissue.)
Related Product Information for anti-PHB antibody
The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in multiple compartments in eukaryotic cells. PHB1 is 30kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3,4). In the nucleus prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2,5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6).
Product Categories/Family for anti-PHB antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PHB (Catalog #AAA330107) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Prohibitin Mouse Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Prohibitin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PHB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prohibitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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