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product-image-AAA215329_SDS_PAGE11.jpg SDS-PAGE (SDS-PAGE Analysis of Purified Proliferation-Marker Mouse Monoclonal Antibody(JC1). Confirmation of Purity and Integrity of Antibody.)

Mouse anti-Human Proliferation Marker Monoclonal Antibody

Proliferation Marker

Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Proliferation Marker, Antibody; Proliferation Marker; anti-Proliferation Marker antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
JC1
Specificity
JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-Proliferation Marker antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
Positive Control
HeLa cells (IF). Human lymph node (IHC).
Immunogen
Pokeweed-stimulated lymphocytes
Cellular Localization
Nucleus
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis of Purified Proliferation-Marker Mouse Monoclonal Antibody(JC1). Confirmation of Purity and Integrity of Antibody.)

product-image-AAA215329_SDS_PAGE11.jpg SDS-PAGE (SDS-PAGE Analysis of Purified Proliferation-Marker Mouse Monoclonal Antibody(JC1). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Lymph Node stained with Proliferation Marker Mouse Monoclonal Antibody (JC1).)

product-image-AAA215329_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Lymph Node stained with Proliferation Marker Mouse Monoclonal Antibody (JC1).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lymph Node stained with Proliferation Marker Mouse Monoclonal Antibody (JC1).)

product-image-AAA215329_IHC15.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Lymph Node stained with Proliferation Marker Mouse Monoclonal Antibody (JC1).)
References
1. Wang et al. 2016. Am J Cancer Res. 6(6):1331-44. 2. Rose, DS et al. J Clin Pathol. 1994 Nov; 47(11): 1010-1014.

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Product Notes

The Proliferation Marker (Catalog #AAA215329) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Proliferation Marker reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Proliferation Marker can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Proliferation Marker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proliferation Marker, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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