ELISA
(Figure-2: An indirect ELISA is carried out by coating Protein A in in serial dilution from 05 ng to 0.078ng and using 100 ng of purified ABM2A4.1E3 monoclonal antibody . Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.)
Mouse Protein A Monoclonal Antibody | anti-spa antibody
Protein A Monoclonal Antibody (Clone: ABM2A4.1E3)
Reactivity
Staphylococcus aureus
Applications
Western Blot, ELISA
Purity
Protein G Chromatography
Synonyms
Protein A, Antibody; Protein A Monoclonal Antibody (Clone: ABM2A4.1E3); Protein A; anti-spa antibody
Host
Mouse
Reactivity
Staphylococcus aureus
Clonality
Monoclonal
Isotype
Mouse IgG2b, kappa
Clone Number
ABM2A4.1E3
Purity/Purification
Protein G Chromatography
Form/Format
Purified; 25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-spa antibody
WB (Western Blot), ELISA
Immunogen
Protein A from Staphylococcus aureus
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles.
ELISA
(Figure-2: An indirect ELISA is carried out by coating Protein A in in serial dilution from 05 ng to 0.078ng and using 100 ng of purified ABM2A4.1E3 monoclonal antibody . Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.)
ELISA
(Figure-2: An indirect ELISA is carried out by coating Protein A in in serial dilution from 05 ng to 0.078ng and using 100 ng of purified ABM2A4.1E3 monoclonal antibody . Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.)
WB (Western Blot)
(Figure-1: Western blot analysis of Protein A. Anti-Protein A antibody (Clone: ABM2A4.1E3) was used at 0.1 ug/ml on Recombinant protein.)
WB (Western Blot)
(Figure-1: Western blot analysis of Protein A. Anti-Protein A antibody (Clone: ABM2A4.1E3) was used at 0.1 ug/ml on Recombinant protein.)
Related Product Information for anti-spa antibody
Protein A (PA) is a 42 kDa surface protein originally found in the cell wall of the Gram positive bacteria Staphylococcus aureus. It has the specific ability to bind with the constant (Fc) portion of immunoglobulin molecules from various different species. Protein A is encoded by the polymorphic X-region and functions as an immunoglobulin-binding protein, characteristically it has the ability to bind with the 'Fc-region' of IgG. Due to its high affinity for Fc region of human IgG, it is extensively used in affinity chromatography process required for the purification of antibodies. However, one of the major disadvantages of this method of purification is co elution or leaching of PA along with the target antibody.
Product Categories/Family for anti-spa antibody
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Product Notes
The spa (Catalog #AAA78565) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Protein A Monoclonal Antibody (Clone: ABM2A4.1E3) reacts with Staphylococcus aureus and may cross-react with other species as described in the data sheet. AAA Biotech's Protein A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the spa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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