Application Data
(Fig.2: SDS-PAGE analysis of purified LT-3D2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LT-3D2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Mouse anti-Human PSA Monoclonal Antibody | anti-KLK3 antibody
anti-human Prostate-Specific Antigen (PSA) monoclonal antibody
Gene Names
KLK3; APS; PSA; hK3; KLK2A1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
PSA, Antibody; anti-human Prostate-Specific Antigen (PSA) monoclonal antibody; anti-KLK3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LT-3D2
Specificity
anti-human PSA (glandular kallikrein 3)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
238
Applicable Applications for anti-KLK3 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human PSA
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.2: SDS-PAGE analysis of purified LT-3D2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LT-3D2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.2: SDS-PAGE analysis of purified LT-3D2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LT-3D2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using LT-3D2 Cat.# GM-0902. BOSC23 cells were transiently transfected with an expression vector encoding either PSA (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LT-3D2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PSA transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using LT-3D2 Cat.# GM-0902. BOSC23 cells were transiently transfected with an expression vector encoding either PSA (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LT-3D2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PSA transfected cells.)
Related Product Information for anti-KLK3 antibody
Background: Prostate specific antigen (PSA) belongs to the kallikrein gene family. It is a single-chain glycoprotein with a molecular mass of about 33 kD which is produced by prostate epithelial cells. It is secreted in the seminal plasma and is responsible for liquefaction of the seminal coagulum (1,2). PSA is present in small quantities in the serum of healthy males, and is often elevated in the presence of prostate cancer and in other prostate disorders. It is one of the most useful tumour markers because of its tissue specificity. It is thus widely used for screening, identification, and monitoring patients with prostate cancer (3).
References
Steven P Balk, Yoo-Joung Ko, Glenn J Bubley (2003). Biology of Prostate-Specific Antigen. Journal of Clinical Oncology 28 (2): 383-91.
Lilja H (2003). Biology of Prostate-Specific Antigen. Urology 62 (5 Suppl 1): 27-33. PMID 14607215.
Oesterling JE (1991): Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate. J Urol 145:907-923
Lilja H (2003). Biology of Prostate-Specific Antigen. Urology 62 (5 Suppl 1): 27-33. PMID 14607215.
Oesterling JE (1991): Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate. J Urol 145:907-923
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,116 Da
NCBI Official Full Name
prostate-specific antigen isoform 3 preproprotein
NCBI Official Synonym Full Names
kallikrein-related peptidase 3
NCBI Official Symbol
KLK3
NCBI Official Synonym Symbols
APS; PSA; hK3; KLK2A1
NCBI Protein Information
prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen
UniProt Gene Name
KLK3
UniProt Synonym Gene Names
APS; PSA; Seminin
UniProt Entry Name
KLK3_HUMAN
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Product Notes
The KLK3 klk3 (Catalog #AAA71456) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Prostate-Specific Antigen (PSA) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the KLK3 klk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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