Mouse anti-Human PSGL-1/CD162 Monoclonal Antibody | anti-PSGL-1/CD162 antibody

Anti-PSGL-1/CD162 Antibody, Mouse Monoclonal

Cat. Num. AAA258912

• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: Protein A
• Synonyms: PSGL-1/CD162, Antibody; Anti-PSGL-1/CD162 Antibody, Mouse Monoclonal; selectin P ligand; Anti-CD162 Antibody; Anti-CLA Antibody; Anti-PSGL-1 Antibody; Anti-PSGL1 Antibody; anti-PSGL-1/CD162 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: #337
• Specificity: Human PSGL-1/CD162
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PSGL-1/CD162 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
• Preparation: This product is a recombinant monoclonal antibody expressed from HEK293 cells.
• Immunogen: Recombinant Human PSGL-1/CD162 Protein
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence staining of SELPLG- in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human SELPLG- monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human CD162 in human tonsil with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining of human CD162 in human spleen with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PSGL-1/CD162 antibody

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

References

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