WB (Western Blot)
(All lanes : Anti-RAB14 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Raji whole cell lysateLane 3: human brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse RAB14 Monoclonal Antibody | anti-RAB14 antibody
RAB14 Antibody
Gene Names
RAB14; FBP; RAB-14
Reactivity
HumanPredicted: Chicken, Mouse, Bovine
Applications
ELISA, Western Blot
Synonyms
RAB14, Antibody; RAB14 Antibody; Ras-related protein Rab-14; RAB14; anti-RAB14 antibody
Host
Mouse
Reactivity
Human
Predicted: Chicken, Mouse, Bovine
Predicted: Chicken, Mouse, Bovine
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1779CT692.32.86
Specificity
This RAB14 antibody is generated from a mouse immunized with a recombinant protein of human RAB14.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
215
Applicable Applications for anti-RAB14 antibody
ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
Human
Cellular Location
Recycling endosome. Early endosome membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle, phagosome. Note=Recruited to recycling endosomes by DENND6A (PubMed:22595670). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-RAB14 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Raji whole cell lysateLane 3: human brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-RAB14 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Raji whole cell lysateLane 3: human brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RAB14 antibody
Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes (By similarity). Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion.
Product Categories/Family for anti-RAB14 antibody
References
Proikas-Cezanne T.,et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases. Ren Y.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases. Huang Y.-P.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases. Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548 (2000). Bechtel S.,et al.BMC Genomics 8:399-399 (2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23897 Da
NCBI Official Full Name
ras-related protein Rab-14
NCBI Official Synonym Full Names
RAB14, member RAS oncogene family
NCBI Official Symbol
RAB14
NCBI Official Synonym Symbols
FBP; RAB-14
NCBI Protein Information
ras-related protein Rab-14
UniProt Protein Name
Ras-related protein Rab-14
UniProt Gene Name
RAB14
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Product Notes
The RAB14 rab14 (Catalog #AAA290839) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAB14 Antibody reacts with Human Predicted: Chicken, Mouse, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's RAB14 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RAB14 rab14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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