Application Data
(Fig.2: CGE analysis of purified BFH-5C9 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified BFH-5C9 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Rat anti-Human Repulsive Guidance Molecule B (RGMB) Monoclonal Antibody | anti-RGMB antibody
Rat anti-human Repulsive Guidance Molecule B (RGMB) monoclonal antibody
Gene Names
RGMB; DRAGON
Reactivity
Human
Applications
ELISA, ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
Repulsive Guidance Molecule B (RGMB), Antibody; Rat anti-human Repulsive Guidance Molecule B (RGMB) monoclonal antibody; anti-RGMB antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a/kappa
Clone Number
BFH-5C9
Specificity
anti-human RGMB
Purity/Purification
Protein G
Form/Format
Phosphate buffered saline, pH 7.2
Sequence Length
478
Applicable Applications for anti-RGMB antibody
ELISA, ELISA, FCM/FACS (Flow Cytometry)
Immunogen
Genetic immunisation with cDNA encoding the extracellular domain (ecd) of human RGMB
Selection
Based on recognition of the ecd region of RGMB on viable, transfected mammalian cells
Preparation and Storage
Short term: Store at 2° to 8°C
Long term: Store at -20°C (avoid repeated freezing and thawing)
Long term: Store at -20°C (avoid repeated freezing and thawing)
Application Data
(Fig.2: CGE analysis of purified BFH-5C9 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified BFH-5C9 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.2: CGE analysis of purified BFH-5C9 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified BFH-5C9 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
FCM/FACS (Flow Cytometry)
(Fig.1: AAA71493 BOSC23 cells were transiently transfected with an expression vector encoding either the ecd of human RGMB (red filled curve), or an irrelevant protein (control transfectant, black curve). Binding of BFH-5C9 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human RGMB.)
FCM/FACS (Flow Cytometry)
(Fig.1: AAA71493 BOSC23 cells were transiently transfected with an expression vector encoding either the ecd of human RGMB (red filled curve), or an irrelevant protein (control transfectant, black curve). Binding of BFH-5C9 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human RGMB.)
Related Product Information for anti-RGMB antibody
Purified Monoclonal Rat Antibody
The RGM domain family member B (RGMB) is a glycosylphosphatidylinositol (GPI)-anchored member of the Repulsive Guidance Molecule family which plays a role in patterning of the developing nervous system (1). There is a potential association between RGMs and cancer bone metastasis, as RGMs coordinate bone morphogenetic protein (BMP) signaling. RGMB may act as a negative regulator in vitro in breast cancer and prostate cancer through BMP signalling (2,3). Furthermore, aberrant expression of RGMs was indicated in breast cancer (4). The perturbed expression was associated with disease progression and poor prognosis. This antibody is recommended for applications under non-denaturing conditions.
The RGM domain family member B (RGMB) is a glycosylphosphatidylinositol (GPI)-anchored member of the Repulsive Guidance Molecule family which plays a role in patterning of the developing nervous system (1). There is a potential association between RGMs and cancer bone metastasis, as RGMs coordinate bone morphogenetic protein (BMP) signaling. RGMB may act as a negative regulator in vitro in breast cancer and prostate cancer through BMP signalling (2,3). Furthermore, aberrant expression of RGMs was indicated in breast cancer (4). The perturbed expression was associated with disease progression and poor prognosis. This antibody is recommended for applications under non-denaturing conditions.
References
1. Severyn CJ, Shinde U and Rotwein P. (2009). Molecular biology, genetics and biochemistry of the repulsive guidance molecule family. Biochem J. 422(3):393-403. doi: 10.1042/BJ20090978.
2. Li J, Ye L, Sanders AJ and Jiang WG (2012). Repulsive guidance molecule B (RGMB) plays negative roles in breast cancer by coordinating BMP signalling. J. Cell. Biochem. 113(7): 2523-2531. doi:10.1002/jcb.24128.
3. Li J, Ye L, Kynaston HG and Jiang WG (2012). Repulsive guidance molecules, novel bone morphogenetic protein co-receptors, are key regulators of the growth and aggressiveness of prostate cancer cells. Int.J. Oncol. 40(2): 544-550. doi: 10.3892/ijo.2011.1251.
4. Li J, Ye L, Mansel RE and Jiang WG (2011). Potential prognostic value of repulsive guidance molecules in breast cancer. Anticancer Res. 31(5):1703-1711.
2. Li J, Ye L, Sanders AJ and Jiang WG (2012). Repulsive guidance molecule B (RGMB) plays negative roles in breast cancer by coordinating BMP signalling. J. Cell. Biochem. 113(7): 2523-2531. doi:10.1002/jcb.24128.
3. Li J, Ye L, Kynaston HG and Jiang WG (2012). Repulsive guidance molecules, novel bone morphogenetic protein co-receptors, are key regulators of the growth and aggressiveness of prostate cancer cells. Int.J. Oncol. 40(2): 544-550. doi: 10.3892/ijo.2011.1251.
4. Li J, Ye L, Mansel RE and Jiang WG (2011). Potential prognostic value of repulsive guidance molecules in breast cancer. Anticancer Res. 31(5):1703-1711.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
RGM domain family member B
NCBI Official Synonym Full Names
repulsive guidance molecule family member b
NCBI Official Symbol
RGMB
NCBI Official Synonym Symbols
DRAGON
NCBI Protein Information
RGM domain family member B
UniProt Protein Name
RGM domain family member B
UniProt Gene Name
RGMB
UniProt Synonym Gene Names
DRAGON
UniProt Entry Name
RGMB_HUMAN
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Product Notes
The RGMB rgmb (Catalog #AAA71493) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat anti-human Repulsive Guidance Molecule B (RGMB) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Repulsive Guidance Molecule B (RGMB) can be used in a range of immunoassay formats including, but not limited to, ELISA, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the RGMB rgmb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Repulsive Guidance Molecule B (RGMB), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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