IF (Immunofluorescence)
(Immunofluorescence staining of PC-3 cell with AAA278998 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 592-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
RGS6 recombinant antibody
RGS6 Recombinant Monoclonal Antibody
Gene Names
RGS6; GAP
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA
Purity
Affinity purified
Synonyms
RGS6, Recombinant Antibody; RGS6 Recombinant Monoclonal Antibody; Regulator of G-protein signaling 6 (RGS6)(S914); RGS6; RGS6 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
12H5
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for RGS6 recombinant antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human RGS6
Conjugation
Non-conjugated
Research Area
Signal transduction
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of PC-3 cell with AAA278998 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 592-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of PC-3 cell with AAA278998 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 592-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IHC (Immunohiostchemistry)
(IHC image of AAA278998 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image of AAA278998 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA278998 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA278998 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
Related Product Information for RGS6 recombinant antibody
Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS6/GNB5 dimer enhances GNAO1 GTPase activity (PubMed:10521509). {ECO:0000269|PubMed:10521509}.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,319 Da
NCBI Official Full Name
regulator of G-protein signaling 6 isoform 1
NCBI Official Synonym Full Names
regulator of G-protein signaling 6
NCBI Official Symbol
RGS6
NCBI Official Synonym Symbols
GAP
NCBI Protein Information
regulator of G-protein signaling 6; GTPase activating protein; H_DJ1108A12.1; S914; WUGSC:H_DJ0283M22.1; WUGSC:H_DJ1108A12.1; regulator of G-protein signalling 6
UniProt Protein Name
Regulator of G-protein signaling 6
UniProt Gene Name
RGS6
UniProt Synonym Gene Names
RGS6
UniProt Entry Name
RGS6_HUMAN
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Product Notes
The RGS6 rgs6 (Catalog #AAA278998) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The RGS6 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RGS6 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the RGS6 rgs6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RGS6, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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