Application Data
(Rabbit anti Human RNA pol II CTD (pThr4) antibody (AAA50469) tested by dot blot analysis. Peptides corresponding to phosphorylated threonine 4 of RNA pol II CTD (lane1), unmodified threonine 4 of RNA pol II CTD (lane 2), phosphorylated serine 2 of RNA pol II CTD (lane 3), and phosphorylated serine 5 of RNA pol II CTD (lane 4) were spotted onto PVDF in decreasing amounts (indicated in picomoles on the left) and probed with AAA50469 at a 1 ug/ml dilution.)
Rabbit anti-Human RNA Pol II CTD Polyclonal Antibody | anti-POLR2A antibody
RNA Pol II CTD (pThr4) antibody
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human
Applications
Western Blot
Purity
Antiserum to human RNA pol II CTD (pThr4) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Synonyms
RNA Pol II CTD, Antibody; RNA Pol II CTD (pThr4) antibody; RABBIT ANTI HUMAN RNA POL II CTD (pThr4); anti-POLR2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antiserum to human RNA pol II CTD (pThr4) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Applicable Applications for anti-POLR2A antibody
WB (Western Blot)
Immunogen
A peptide containing the RNA pol II C-terminal sequence phosphorylated at threonine 4
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Rabbit anti Human RNA pol II CTD (pThr4) antibody (AAA50469) tested by dot blot analysis. Peptides corresponding to phosphorylated threonine 4 of RNA pol II CTD (lane1), unmodified threonine 4 of RNA pol II CTD (lane 2), phosphorylated serine 2 of RNA pol II CTD (lane 3), and phosphorylated serine 5 of RNA pol II CTD (lane 4) were spotted onto PVDF in decreasing amounts (indicated in picomoles on the left) and probed with AAA50469 at a 1 ug/ml dilution.)
Application Data
(Rabbit anti Human RNA pol II CTD (pThr4) antibody (AAA50469) tested by dot blot analysis. Peptides corresponding to phosphorylated threonine 4 of RNA pol II CTD (lane1), unmodified threonine 4 of RNA pol II CTD (lane 2), phosphorylated serine 2 of RNA pol II CTD (lane 3), and phosphorylated serine 5 of RNA pol II CTD (lane 4) were spotted onto PVDF in decreasing amounts (indicated in picomoles on the left) and probed with AAA50469 at a 1 ug/ml dilution.)
WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (40 ug) probed with RNA pol II CTD (pThr4) antibody (AAA50469) at a 2 ug/ml dilution.)
WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (40 ug) probed with RNA pol II CTD (pThr4) antibody (AAA50469) at a 2 ug/ml dilution.)
Related Product Information for anti-POLR2A antibody
Rabbit anti Human RNA pol II CTD (pThr4) antibody recognizes RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at threonine 4 of the carboxyl-terminal repeat domain.
RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999) (Sims et al. 2004). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle (Phatnani and Greenleaf, 2006). Phosphorylation of the CTD of RNA pol II at threonine 4 has been shown to facilitate histone mRNA 3' end processing (Hsin et al. 2011). Phosphorylation of the carboxyl-terminal repeat domain of RNA polymerase II at threonine 4 occurs at the 3' end of most human genes and is necessary for transcription elongation (Hintermair et al. 2012).
Wide species cross-reactivity is expected from Rabbit anti Human RNA pol II CTD (pThr4) based on sequence.
RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999) (Sims et al. 2004). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle (Phatnani and Greenleaf, 2006). Phosphorylation of the CTD of RNA pol II at threonine 4 has been shown to facilitate histone mRNA 3' end processing (Hsin et al. 2011). Phosphorylation of the carboxyl-terminal repeat domain of RNA polymerase II at threonine 4 occurs at the 3' end of most human genes and is necessary for transcription elongation (Hintermair et al. 2012).
Wide species cross-reactivity is expected from Rabbit anti Human RNA pol II CTD (pThr4) based on sequence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
217,176 Da
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Official Symbol
POLR2A
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase II largest subun
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1
UniProt Entry Name
RPB1_HUMAN
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Product Notes
The POLR2A polr2a (Catalog #AAA50469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNA Pol II CTD (pThr4) antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNA Pol II CTD can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the POLR2A polr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA Pol II CTD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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