WB (Western Blot)
(Western blot analysis of 15 ug cell lysates from DH5α and BL21 cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 5 ug/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. please inquire). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. please inquire) was used for developing.)
Mouse anti-E Coli RNA Sigma 32 Monoclonal Antibody
Purified anti-E. coli RNA Sigma 32 Antibody
Reactivity
E Coli
Applications
Western Blot
Synonyms
RNA Sigma 32, Antibody; Purified anti-E. coli RNA Sigma 32 Antibody; SigmaH; rpoH; fam; hin; htpR; Heat shock regulatory protein F33.4; RNA Polymerase; anti-RNA Sigma 32 antibody
Host
Mouse
Reactivity
E Coli
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
3RH3
Form/Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/ml (varies by lot)
Applicable Applications for anti-RNA Sigma 32 antibody
WB (Western Blot)
Immunogen
Recombinant protein
Recommended usage
Each lot of this antibody is quality control by Western Blotting. For Western blotting. the suggested use of this reagent is 0.5-5.0 ug/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Structure
Sigma 32(a 32 kD) transcription factor of E.coli RNA polymerase.
Biology Area
Cell Biology,Transcription factors.
Molecular family
Nuclear Markers.
Preparation
The antibody was purified by affinity chromatography.
Preparation and Storage
The antibody solution should be stored undiluted between 2°C and 8°C.
WB (Western Blot)
(Western blot analysis of 15 ug cell lysates from DH5α and BL21 cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 5 ug/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. please inquire). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. please inquire) was used for developing.)
WB (Western Blot)
(Western blot analysis of 15 ug cell lysates from DH5α and BL21 cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 5 ug/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. please inquire). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. please inquire) was used for developing.)
WB (Western Blot)
(Western blot analysis of 15 ug cell lysates from DH5α cells. Samples were incubated at 37°C for 0, 5, 10, 15, and 40 minutes and then resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 2 ug/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. please inquire). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. please inquire) was used for developing.)
WB (Western Blot)
(Western blot analysis of 15 ug cell lysates from DH5α cells. Samples were incubated at 37°C for 0, 5, 10, 15, and 40 minutes and then resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 2 ug/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. please inquire). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. please inquire) was used for developing.)
Related Product Information for anti-RNA Sigma 32 antibody
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters.
References
Lesley SA, et al. 1987. J. Biol. Chem. 262:5404.
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Product Notes
The RNA Sigma 32 (Catalog #AAA78935) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Purified anti-E. coli RNA Sigma 32 Antibody reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's RNA Sigma 32 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the RNA Sigma 32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA Sigma 32, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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